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T1 Phage-Resistant E. coli DH5α? T1 Phage-Resistant E. coli OmniMAX2 T1 Phage-Resistant Cells Rosetta Rosetta(DE3) Rosetta(DE3) pLysS Rosetta2 (DE3) pLysS BL21(DE3)gold BL21(DE3)star Origami(DE3) Origami2(DE3) Rosetta-gami 2(DE3)pLysS, Rosetta-gami B(DE3)pLysS ArcticExpress? (DE3)RP BL21 (DE3)gold-pRK1037 35S::GFP 35S::dsRed 35S::mCherry pUbi::GFP pUbi::GUS pLH6000 pC1301s DS1301 Bacillus subtilis 168 Bacillus subtilis 1A75 Bacillus subtilis WB600 Bacillus subtilis WB800 PMUTIN4 PSG1154 PHCMC05 PMA5 MCS PHT304 MCF-7 K562 T2A2 B16 pASK101 Bacillus subtilis 1A75 PMA09-wapA-SP H3 PMA09-yncM-SP J3 pGFP22 pGFP315 pHT43 pWB980 pAX01 pMA5 MCS pRS426 pCSN44 FY834 pIL253 pEGM-3ZF(+) PUCM-T P3-neo-loxp打靶载体 pShuttle-IRES-hrGFP-2 35S::GFP 35S::dsRed 35S::mCherry pUbi::GFP pUbi::GUS pLH6000 pC1301s DS1301 pUBIN-ZH2 pActin ArcticExpress? (DE3)RP Mach1? T1 Phage-Resistant E. coli DH5α? T1 Phage-Resistant E. coli OmniMAX2 T1 Phage-Resistant Cells pAYCYDuet-1 pETDuet-1 pRSFDuet-1 pCDFDuet-1 pCold I pCold TF pG-KJE8 pGro7 pKJE7 pG-Tf2 pTf16 pME6032 pKT100 pZL507 DH5a BL21(DE3) PA01(铜绿假单胞杆菌) YPIII(假结核鼠疫耶尔森氏菌) BL21(DE3) JM109(DE3) 枯草芽孢杆菌168 AS1.1391 产酸克雷伯士菌 W103 产气肠杆菌 副干酪乳杆菌HD1.7 米曲霉 休哈塔假丝酵母20335 HDYXHT-01 酿酒酵母W5 大肠杆菌DH10Bac 恶臭假单胞菌ATCC12633 莓实假单胞菌ACCC03879 恶臭假单胞菌ACCC10162 CICC1766 农杆菌EHA105 pMG36e pHSG415 PYC6 P406ADH1 p2NIL PRSFDuet-1 pet32a,pet28a, pet27b, pklight Pgex pee12.4,pee6.4 PBI121 pcDNA3.0,PNZ. Pfastbac pVAX1-optiF pGWB2 pENTR 杂交系统: 哺乳动物双杂交系统 Promega Vectors:pACT,pBIND Contorl plasmid:pACT-MyoD,pBIND-Id Reporter plasmid:pG5 luc Stratagene Vectors:pCMV-BD,pCMV-AD Contorl plasmid:pBD-p53,pBD-NF?κB,pAD-SV40T,pAD-TRAF Reporter plasmid:pFR-luc 酵母双杂交系统 Clontech Vectors:pGBKT7,pGADT7, Positive contorl:pCL1,pGBKT7-53,pGADT7-T Negative control:pGBKT7-Lam Strain:AH109 Vector:pACT2 AD Stratagene Vectors:pSos,pMyr Control plasmid:pSos MAFB, Strain: 酵母单杂交系统 Clontech Vector:pHis2 酵母三杂交系统 Clontech Vector:pBridge Strains:Y2H Gold Yeast strain,Y187 诱导系统: 蜕皮激素诱导表达系统 Invitrogen Vectors:pIND,pVgRxR LacSwith II哺乳动物诱导表达系统 Stratagene Vectors:pOPRSVI,pOPI3CAT,pCMVLacI GeneSwith 哺乳动物诱导系统 Invitrogen Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI) 四环素诱导系统 Invitrogen Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C, Contorl plasmid:pcDNA4/TO/Myc-His/LacZ Regulatory plasmid:pcDNA6/TR Clontech Vectors:pTet-On,pTet-Off,pTRE2,pTK-hyg pRevTet-On,pRevTet-Off,pRevTRE pTet on advanced,pTRE Tight,pTRE Tight Luciferase pCMV-Tet3G,pTRE3G,pTRE3G-Luc, 腺病毒系统: Stratagene Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1 Strains:BJ5183,AdEasy-1 Invitrogen Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector, 腺相关病毒系统: Stratagene Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP, pCMV-MCS 逆病毒系统: RNAi Clontech Vectors:RNAi-Ready pSIREN-RetroQ, RNAi-Ready pSIREN-RetroQ-DsRed-Express RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide), 基因表达载体 Clontech Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro, pRetroX-IRES-DsRed Express Vectors:pLVTH,pSuper retro puro,pBabe puro 逆病毒包装载体 Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho 慢病毒系统: RNAi载体 Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G, pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3 Clontech Vectors:pLVX-shRNA1,pLVX-shRNA2 SBI Vectors:pSIH1-H1-CoGFP Open Biosystems Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control 基因表达载体 Clontech Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1 pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro, pLVX-Tight-Puro,pLVX-Tet-On-Advanced Invitrogen Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST Vector:pWPXL,FUGW 慢病毒包装载体 Invitrogen Vectors:pLp1,pLp2,pLp VSV G Strain:Stbl3,Stbl4 Cell biolabs Vectors:pCgpV Clontech Vector:pVSV G Second generation: Vectors:pCMV-dR8.91,pCMV-VSV-G Second generation: Vectors:pMD2.G,pSPAX2 Third generation: Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G iPS慢病毒载体 Cell biolabs Vectors:pLentG-KOSM Vectors:FUW-M2rtTA,TetO-FUW-OSKM PiggyBac转座子系统 Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector), PiggyBac 3’LTR (pMD18T vector) 信号通路报告系统: Clontech Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc, pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc pCRE-Luc,pGRE-Luc,pHSE-Luc Stratagene Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc, Promega Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19 Millipore Vectors:TOPFlash,FOPFlash,SuperTopFlash RNAi系统: Ambion Vectors:pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro Reporter plasmid:pMIR-REPORT Luciferase Promega Reproter plasmid:psiCHECK-2,pmir GLO Oligoengine Vector:pSuper-puro Other Vector:pGenesil-1 Clontech Vector:pmR mCherry SBI Vector:pmiRZip anti-microRNA 酵母表达系统: 酿酒酵母 Invitrogen Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor, Strain:INVSc1 毕赤酵母 Invitrogen Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C,pPIC9K,pPIC3.5K,pPICZ A,pPICZ B,pPICZ C, Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168, Invitrogen Vector:pPink HC,pPink LC Strain:Pichiapink-1,Pichiapink-4 Gateway系统: Invitrogen Vectors:pENTR 1A,pENTR3C,pDONR221,pcDNA6.2-GWEmGFP-miR negative, Regulatory plasmid:pLenti6/TR Strain:DB3.1 表面展示系统: 酵母表面展示 Invitrogen Vector:pYD1 Strain:EBY100 哺乳动物表面展示 Invitrogen Vector:pDisplay 噬菌体表面展示 Vector:pCantab 5e Strain:HB2151,TG1,M13KO7(in TG1) 重组蛋白乳汁表达系统: Invitrogen Vector:pBC1 绝缘子检测系统: Invivogen Vector:pWHERE 果蝇细胞表达系统: Invitrogen Vector:pAc5.1 V5-His B,pCo hygro 哺乳动物表达系统: Invitrogen Vectors:pcDNA3.1(neo)+,pcDNA3.1(neo)-,pcDNA3.1(hyg)+,pcDNA3.1(zeo)+, pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax B, pcDNA6-Myc/His B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2, pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ, pSFV1,pEF1/myc-His B,pcDNA3.1(+)/Myc HisB,pEF6/Myc-HisC, Stratagen Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B,pBK-CMV,pIRES-hrGFP-1a Promega Vectors:pCI-neo,pCI,pSI Clontech Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES hyg3, pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen), pIRES2-EGFP,pIRES puro2 Sigma Vectors:pFLAG CMV2 Invivogen Vectors:pVitro2-neo-mcs Vectors:pCAGGS 昆虫细胞表达系统: Invitrogen Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B, pBlueBacHis2 C,pVL1392,pVL1393, Strain:DH10Bac Vectors:pIZT/V5-His,pMIB v5-His B Cell:sf9,High Five MultiBac Expression System Vectors:pFBDM,pUCDM,pBADZ-His6Cre, Strains:DH10MultiBac,BW23473 Cell:sf9,High Five 荧光蛋白报告系统: Clontech Vectors:pDsRed1-C1,pDsRed2-N1,pDsRed2-C1, pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pECFP-Mito,pLEGFP-N1,pLEGFP-C1,pDsRed2-1,pEGFP-C3 FRET assay Vectors:Dkeima fluorescent protein,mAmetrine, 启动子增强子报告系统: Promega Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter, pRL-TK,pRL-CMV,pRL-SV40 NEB Vectors:pGluc-basic 原核表达系统: Novagen Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+), pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+), pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+),pET49b(+),pET50b(+), pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1 Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS, Rosetta-gami B(DE3)pLysS,BLR(DE3),BL21(DE3)gold,Rosetta Blue(DE3) Takara Vectors:pCold I,pCold TF NEB Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x Biosee Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx, pET-His,pTrc-CKS,pET-DsbA Stratagene Strain:BL21 codonplus(DE3)RIPL, Invitrogen Strain:BL21(AI) Qiagen Vector:pQE30 Strain:M15 NEB Vectors:pTWIN1,pTYB11,pMXB10,pTXB1 Strain:ER2566 Amersham Vectors:pGEX-4T-1,pGEX-5X-2,pGEX-6P-1,pGEX-2TK Invitrogen Vectors:pBADHis A,pBADHis B,pBADHis C, Other Vector:pBV220,pBluescript II SK(+) Takara 分子伴侣系统 Vector:pG-KJE8,pGro7,pKJE7,pG-Tf2,pTf16, 基因载体: Invitrogen Vector:pOG44 Invivogen Vectors:pORF-lacZ,pORF-HSV1tk 克隆载体: Promega Vectors:pUC18,pUC19,pBR322,pSP64,pSP73 柯斯载体: Stratagene Vector:SuperCos1 同源重组载体: Vector:pSIM5,pSIM6 克隆菌株: Stratagene Strains:DH5α,JM109,JM110,TOP10,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1 Phage-Resistant Cells,XL10 Gold,EZ10,DH10B,SURE, 基因: Genes: human TERT,Taq,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mous pcDNA3.2/V5-DEST ;pcDNA6/myc-His A&B&C ;pcDNA3.1(-)/myc-His A&B&C;pcDNA3.1/GS;pcDNA3.1/Zeo(+);pTracer CMV2;pBudCE4.1;pVAX1;pSecTag2 A&B&C;pSecTag A&B&C;pCDNA4/HisMax A&B&C;pcDNA3.1/V5-His A&B&C;pcDNA3.1(+)/CAT;pcDNA3.1-EGFP;pSilencer1.0;pSilencer 2.1-U6 hygro;pSilencer 3.0-H1;pSilencer 3.1-H1 hygro;pSilencer 3.1-H1 neo;pSilencer 4.1-CMV neo;pSilencer 4.1-CMV puro;pSilencer5.1-U6 Retro;pSilencer5.1-H1 Retro;pET-5a(+);pET-11a(+);pET-11b(+); FLAG-TRAF2 87-271 FLAG-TRAF2 1-271 FLAG-TRAF2 1-87 HA-GSTPi WT FLAG-Mtraf6 FL XP-JNKK2 WT NF-Kb luc JUN2-luc HA-GSTPi 2 突变体 JNKK2 WT HA-ASK WT PC-DNA3.1 FLAG-TRAF6 MEKK1 FL.A MEKK1 FL.B MEKK1-△FLAG C-MYC ASK FL MKK6 DD P38 WT A PC-DNA3 FLAG-TRAF2 FL TRAF2 △N HA-Ub TRAF6-C FLAG-TRAF2 272-501 HA-MEKK1 HA-GSTPi FLAG-Mtraf6-△N PCMV-FLAG HSP27 S78/82A PCMV-FLAG HSP27 S15A PCMV-FLAG HSP27 (WT) HA-HSP90a HA-MKK3 WzG PCDNAsFLAG GS WT HA-HSP90b PBS-V6 IKKb PCMV HA 2×HA GSTPi Cys 3变 Myc-CHIP iNOS-luc 1.3kb U6-CHIP PCMV-HA-TRAF6 PCMV-Myc-TRAF6 ERK WT HA-SUMO STAT1 Report PET28 GSTPi WT90(克隆) GS-PET28 BL281(表达) PET 3C gln A DH5a BL21 PET28(质粒) PET3C/B BL21(DE3) M15 PQE/Blys XL1 PET 43.1b(+) DNA PET 30a-Blys Kan r BL21(DE3)JNKK2 BL21 PET 28a JNKK2(KM) DH5a PET 28 WT JNKK2 DH5a PET 28a DH5a PET28 JNKK2(A) DH5a PET28 JNKK2 XL-1 PET43b BL21 PET28 GS BL21 PET28a GS BL21-Gold (DE3) 菌 BL21-Gold (DE3)-plyss BL21-Goldonplus(DE3)-RIPL BL21-PET 28 JNKK2 WT PET 30a-Blys PET30 XP-JNKK2 WT HA-Ub XP-HSP70 FLAG-TAK1 PCDNA 3.1-FLAG TAK1 DH5a TRAF2 87-271 HA-GSTPi WT Xpress GSTPi TWAL B Xpress GSTPi TWAL A XP-GSTPi 85-210 B XP-GSTPi 85-210 A XP-GSTPi 1-84 B XP-GSTPi 1-84 A XP-GSTPi-YST B XP-GSTPi-YST A PCDNA 3 B C-mys ASK FL D C-mys ASK FL E TRAF2 1-271 TRAF2 272-501 JUN-luc HA-JNK1 HA-JNK2 TRAF2 87-501 FLAG-TRAF2 1-271 HA MEKK2 FL HA MEKK1 KM XP-JUKK2 WT HA-MKK3 HA-MKK6 HA-MEKK1 FLAG-TRAF2 △N PGFP3-HSP25 HA-T6RZ HA-T6RZC Ub ( K63) BL21 DE3 XL-1 Blue PET 28a GFP XP-GCK DH5a(菌) PGFP1 HSP25 PGEFP3 HSP25(菌) XP-JNKK2 KM DH5a GSTPi 无tag FLAG-TRAF2 HA-GSTPi 四突变 DH5a FLAG-TRAF (272-501) HA-GSTPi 三突变 GSTPi Y7 HA-TAB2 DH5a HA-TAB1 FLAG-JNK1 DH5a HA-ASK1 FLAG-ASK1 P53 △81 DH5a-Psuper Vector P21-luc WAF HA-IKBa GST-ATF TRAF6-DH5a HA-TRAF2 FLAG-TRAF2 GST TRAF6 coil GST AIF DH5a HA-IKBa PGEX-4T1 GST IKB TAK1 K63A P53 WT GSTPi △C GSTPi WT B-gal DH5a 5×JUN2 luc P38 JSF HA-GSTPi 2突变 HA-GSTPi WT SM MKK4 PCMV-FLAG-HSP27 PCDNA3 XP-HSP27 FL P38 WT A MKK4 △N HSP70 ASK1 sense FLAG MKK7 b1 PCMV-FLAG-HSP27 JNK-APF HA-JNK FLAG-MEE2.1 FLAG-MKK2 2 PCDNA3-FLAG-HSP27 GSTPi TWAL HA-TRAF6 Myc-TRAF2 SOD-DH5a FLAG-HSP70 FLAG-TRAF6 WT FLAG-MKK7 Dh5a pET28 GSTpi 1-84 Dh5a pET28 GSTpi 85-210 DH5a pcDNA3 HA-GSTpi 85-210 pcDNA3 HSp27 HA-GSTpi Y7S BL21-pET28 GSTpi Y7S BL21-pET28 GSTpi 1-84 BL21-pET28 GSTpi 85-210 pGEX-4T-1 MEKK1 HSP27 HSP70 HA-Hsp90a HA-HSp90b DH5a FLAG-GST pTRE2 HA-GSTp DH5a pTRE2 HA-GSTp XL-1 Blue pET28a GSTpi WT iNOS-luc 1.3kb pET43b pET28a pET28-Hsp27 BL21 DH5a pcMV Flag MEKK1 Flag MKK1 a1 ASK1 WT no tag TRAF2 FL MKK4 ΔN p38 no tag MEKK1 871 MKK7 a2 Flag HA-ASK1 FL TRAF2 1-271 MKK4 WT XP-JNKK2 KM ΔMEKK1 no tag FLAG MKK7 β1 HA ASK1 ΔC TRAF2 87-271 HA-TRAF6 WIG XP JNKK CA HA-MKK3 Flag TAK1 B TRAF2 ΔN HA-ASK1 ΔN Flag-MKK7 β2 MEKK1 HA FL HA MEKK1 FL2 FLAG MKK7 P1 ASK1 KN TRAF2 272-501 FLAG TAK1 β1 HA-MKK6 HA-MEKK1 CA FLAG MKK7 P2 XP-GCK HA-MEKK2 FL XP-JNKK2 WT HA-MEKK1 KM AP-1 LUC HA-MEKK2 KM FLAG-HSP70 PCDNA3 STAT1 report p21 LUC XP-HSP21 FL12/25 Jun2 LUC STAT3 LUC FLAG-TRAF6 ΔN HA-GSTpi 3变 HA-GSTpi WT FLAG-HSP27 GSTpi HA 4变 HA-GSTpi 47/101 GSTpi TRC no tag GSTpi no tag2 β-gal2 β-gal1 GSTpi WT no tag GSTpi Y7S no tag GSTpi WT P38 ASP P38 β WT P38 DN MEKK1(CA) MEKK1 KM MEKK1 HA (KM) MEKK1-f1-HA PSRα HA-JNK1 FLAG JNK1 WT JNKK2 KM JNKK2 CA JNKK2 WT ΔMEKK1 PCDNA MKK4 SEK NL gal 1 JNK JNK APF ASK1 WT HA-ASK1 ASK1 DC-HA ASK1-ΔN-HA ASK KN HA MEKK2 KM NF-KB HA-MEKK2 FL MKK3 MKK6 PCDNA GSTp Y7 GSTp Δc GSTp HA PCDNA MKK7 α1 PCDNA MKK7 α2 PCDNA MKK7 β1 PCDNA MKK7 β2 PCDNA MKK7 P1 PCDNA MKK7 P2 AP-1 LUC 5X P85 Δc P110 α P110 Δ Akt WT Akt(D) P85 N FLAG 27 S15A FLAG 27 WT FLAG 27 S78/82A PCDNA3 c-Jun 73A PCMV c-Jun 73D xp-GCK STAT3 STAT1 GST MU IKB ΔN gal Bcl-2 Hyg IKKβ pcDNA FLAG-TAK1 TRAF6 HA-GST pGFP3 hsp25 FLAG JNK-MU XL-1 FL MEKK1 FLAG △M MKK 6E MKK 3E MKK 4A MKK 4E p38β Af Red ATF α PET43a Flag-△MEkk1 HA-T6RE BT5183 XP-JNKK2 WT Flag-TLR4 pcDNA-Flag-His pcDNA-myc-His Ikk β Ik β △TRAF 6 pcDNA myc TRAF6 FL pcDNA HA pcMV Flag p21-luc GST-4变 c-myc-ASK1 FL c-myc-ASK1 FL Flag-JNK Flag-JNK(mu) MKK6 DD HA-JNK2 Flag-JNK1 HA-JNK1 Flag-Ask1 TRAF2 87-271 TRAF2 △N TRAF2 272-501 TRAF2 1-271 HA-Mkk3 HA-Mkk6 F-TRAF2 272-底 F-TRAF2 1-271 F-TRAF2(FL) F-TRAF2 87-271 F-TRAF2 △N His ubc B His uev 1A Flag p21 Flag-TRAF6 291-358 WC PCMV-HA-TRAF6 WT Flag-TRAF6 1-358 DM Pgex GST-IkB WT HA-TbRE Flag-TRAF3 1-391 WC Flag-TRAF6 1-358 WC pGEX GST-ATF2 HA-TbREc GST-TRAF6(coil-coil) TRAF6 1-358 TRAF6 △292-358 His-Ubc 13 TRAF6 1-291 psuper mull 2 Ub.E.coli NF kB luc P-Super Hsp27 RNAi P-Super null HA-T6RE Flag-TR6F6 △C.C Flag-TR6F6 289-360 Flag-TR6F6 255-530 Flag-TR6F6 108-269 Flag-TR6F6 289-530 Flag-TR6F6 108-530 Flag-TR6F6 1-108 Flag-TR6F6 △C His-Ucr1A kan HA-TBRZ C myc-△MEKK 1 myc-MEKK 1 Flag-△MEKK1 FL-Rmekk1 XP-Hsp70 △ABD XP-Hsp70 421-642 XP-Hsp70 121-642 XP-Hsp70 △C 1-420 XP-Hsp70 1-120 XP-Hsp70 121-420 pCMV-MEK1 GSTPi RNAi MKK6 △ IkB Stat 3 pcDNA HA-His Flag-TLR4 pFlag-MV2 pcDNA myc-His mTRAF6 △N.Flag pTRE 2 myg PSRed N1 Flag p38(AF) Flag p38 WT Flag p38.(AF) △MEKK1 HA-Mkk4 HA-MKK3 HA-MKK6 Flag-ERK WT Flag-p38 d WT HA-Ub pCMV-ATF2 Flag-p38 β WT Flag ERK2( r ) Flag-p38 δ WT PET28a 原始 PET28a 空1 PET28a 空 PET28 GSTpi WT PET28 GSTpi YTS PET28 GSTpi TWAL PET28 GSTpi WT A PET28 JNKK2 CA PET28 JNKK2 KM PET28 JNKK2 WT pcDNA3 Flag-GSTpi YTS PET28 GSTpi 1-84 A1 PET28 GSTpi 1-84 T PET28 GSTpi 85-210 B1 PET28 GSTpi85-210 B2 pcDNA3 HA-GST 85-210 pcDNA3 HA-GST 85-210 pcDNA3 HA-GST 1-84 PTRE2 Hyg 空 pcDNA3 Flag-TRAF2 1-87 Xpress GSTpi WT Xpress GSTpi YTS B Xpress GSTpi 1-84 B Xpress GSTpi YTS A Xpress GSTpi 85-210 B Xpress GSTpi TWAL B pcDNA3 HA-GST 1-84 pcDNA3 HA-GST 85-210 MKK7α2 纯 MKK7β1 MKK4 △MEKK1 GST wt 纯GSTP MV pcDNA a pcDNA b 2×HA GSTP Cys 4变 2×HA GSTP Cys 2变 2×HA GSTPi TWAL Xpress GSTPi YTS Xpress GSTPi TWAL Xpress GSTPi WT HA GSTP WT Flag GSTP WT GSTpi TRC△8 no tag GSTpi YTS no tag 2×HA JNK WT XP-JNKKα WT XP-JNKKα Xpress-JNKKα KM Xpress-JNKKα CA JNK-luc iNOS-luc NFkB-luc Flag-TRAF6 △N Flag-TRAF6 FL PCMV Myc-TRAF6 HA-TRAF6 Flag-TRAFα FL PCMV myc-TRAFα WT Flag-TRAFα 272-501 Flag-TRAFα △N P38 no tag Flag-p38 β Flag-p38 α WT HA-ASK1 △N HA-ASK1 FL HA-ASK1 FL Flag-TAK1 Flag-TLR4 Flag-TAK1(K63A) HA-MEKK1 FL Rat-MEKK1FL Flag-Mkk7α1 Flag-Mkk7β1 Flag-Mkk7 Mkk6 HA-Mkk6 HA-MEKK2 FC pcDNA3 Flag-Hsp27 pcMV Flag-Hsp27 S78/82 pcMV Flag-Hsp27 S/5A Xpress Hsp70 Hsp70 antisense XP-Hsp27 pGFP-Hsp25 pcDNA3 Flag-Hsp25 STAT3 report pcDNA3 Flag-GFP AP-1 luciferase Flag-GFP Flag-P21 Flag-TRAF6 1-358 Flag-TRAF6 wt Flag-TRAF6 Δ291-358 Flag-TRAF6 ΔN HA-CHIP HA-hsp27 wt HA-hsp27 Δ15A HA-hsp27 Δ78/82A HA-P53 wt HA-TRAF6 wt HA-Ub Jun-2 luciferase MKK7 Myc-hsp27 wt Myc-hsp27 Δ15A Myc-hsp27 Δ78/82A NF-kB luciferase P21 luciferase PCMV-pcDNA PET282 JNK2 PET28a JNK1 ASKc Flag-GCS Flag-GFP Flag-Gs Flag-GSTPi Flag-Hsp27 Flag-hsp27 Flag-Hsp70 Flag-JNK1-GFP Flag-JNK2 mut Flag-JNK2-GFP Flag-pcDNA3 Flag-ΔMEKK1 GFP(无Tag) GST-red2 HA-ASK1(1-498) HA-GlaR HA-GS HA-GST wt HA-GST-2变 HA-GST-3变 HA-GST-4变 HA-GST-4变 HA-GSTPi HA-Hsp90a HA-hsp90β HA-JNK1 HA-MKK3 Hsp70-antisense JNK1-red2 JNK-APF JNK-EGFP MEK-EL P21-GFP p53 p53-Δ81 pcDNA3-2*HA-JNK2 pcDNA3-GFP pcDNA3-GFP-JNK2 pcDNA3-GST-GFP pcDNA3-JNK1-GFP pcDNA3-JNK2-EGFP pcDNA-Flag-GCS pcDNA-GFP(单) pcDNA-GFP(双) pcDNA-p53-HA PET28a PET28-GFP PSR-HA-JNK1 TNK1-GFP TNK-APF x xp-GST xp-GST-Y7F xp-Hsp27 XP-hsp70 xp-hsp70 A段 xp-hsp70 wt xp-hsp70-AC xp-Hsp70-B xp-hsp70-wt xp-Hsp70-ΔC xp-hsp70-ΔN xp-ΔN-HSP70 pSUPER90i(amp+) pcuv pcDNA HA-cdc37 HA-T6R2 HA-T6R2C HA-HSP90a HA-HSP90a(1-401aa) HA-HSP90a(401-620aa) HA-HSP90a(1-620aa)表达不好 Flag-TAK1 Psuper HA-ubiputin AP-Luciferase TAB1 Flag-GFP Flag-TAK1(1-299aa) Flag-TAK1(301-579aa) HA-HSP90a(1-232aa) HA-HSP90a(233-732aa) HA-HSP90a(402-732aa) Akt(D) Akt(wt) MEK-EL pcDNA3 BL-21-ΒET-3C-TRAIL及4种变体 BL21(DE3) JM109(DE3) 枯草芽孢杆菌168 AS1.1391 产酸克雷伯士菌 W103 产气肠杆菌 副干酪乳杆菌HD1.7 米曲霉 休哈塔假丝酵母20335 HDYXHT-01 酿酒酵母W5 大肠杆菌DH10Bac 恶臭假单胞菌ATCC12633 莓实假单胞菌ACCC03879 恶臭假单胞菌ACCC10162 CICC1766 农杆菌EHA105 pMG36e pHSG415 PYC6 P406ADH1 p2NIL PRSFDuet-1 pet32a,pet28a, pet27b, pklight Pgex pee12.4,pee6.4 PBI121 pcDNA3.0,PNZ. Pfastbac pVAX1-optiF pGWB2 pENTR Bacillus subtilis 1A75 PMA09-wapA-SP H3 PMA09-yncM-SP J3 pGFP22 pGFP315 pHT43 pWB980 pAX01 pMA5 MCS pRS426 pCSN44 FY834 pIL253 pEGM-3ZF(+) PUCM-T P3-neo-loxp打靶载体 pShuttle-IRES-hrGFP-2 35S::GFP 35S::dsRed 35S::mCherry pUbi::GFP pUbi::GUS pLH6000 pC1301s DS1301 pUBIN-ZH2 pActin ArcticExpress? (DE3)RP Mach1? T1 Phage-Resistant E. coli DH5α? T1 Phage-Resistant E. coli OmniMAX2 T1 Phage-Resistant Cells pAYCYDuet-1 pETDuet-1 pRSFDuet-1 pCDFDuet-1 pCold I pCold TF pG-KJE8 pGro7 pKJE7 pG-Tf2 pTf16 pME6032 pKT100 pZL507 DH5a BL21(DE3) PA01(铜绿假单胞杆菌) YPIII(假结核鼠疫耶尔森氏菌) BL21(DE3) JM109(DE3) 枯草芽孢杆菌168 AS1.1391 产酸克雷伯士菌 W103 产气肠杆菌 副干酪乳杆菌HD1.7 米曲霉 休哈塔假丝酵母20335 HDYXHT-01 酿酒酵母W5 大肠杆菌DH10Bac 恶臭假单胞菌ATCC12633 莓实假单胞菌ACCC03879 恶臭假单胞菌ACCC10162 CICC1766 农杆菌EHA105 pMG36e pHSG415 PYC6 P406ADH1 p2NIL PRSFDuet-1 pet32a,pet28a, pet27b, pklight Pgex pee12.4,pee6.4 PBI121 pcDNA3.0,PNZ. Pfastbac pVAX1-optiF pGWB2 pENTR 杂交系统: 哺乳动物双杂交系统 Promega Vectors:pACT,pBIND Contorl plasmid:pACT-MyoD,pBIND-Id Reporter plasmid:pG5 luc Stratagene Vectors:pCMV-BD,pCMV-AD Contorl plasmid:pBD-p53,pBD-NF?κB,pAD-SV40T,pAD-TRAF Reporter plasmid:pFR-luc 酵母双杂交系统 Clontech Vectors:pGBKT7,pGADT7, Positive contorl:pCL1,pGBKT7-53,pGADT7-T Negative control:pGBKT7-Lam Strain:AH109 Vector:pACT2 AD Stratagene Vectors:pSos,pMyr Control plasmid:pSos MAFB, Strain: 酵母单杂交系统 Clontech Vector:pHis2 酵母三杂交系统 Clontech Vector:pBridge Strains:Y2H Gold Yeast strain,Y187 诱导系统: 蜕皮激素诱导表达系统 Invitrogen Vectors:pIND,pVgRxR LacSwith II哺乳动物诱导表达系统 Stratagene Vectors:pOPRSVI,pOPI3CAT,pCMVLacI GeneSwith 哺乳动物诱导系统 Invitrogen Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI) 四环素诱导系统 Invitrogen Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C, Contorl plasmid:pcDNA4/TO/Myc-His/LacZ Regulatory plasmid:pcDNA6/TR Clontech Vectors:pTet-On,pTet-Off,pTRE2,pTK-hyg pRevTet-On,pRevTet-Off,pRevTRE pTet on advanced,pTRE Tight,pTRE Tight Luciferase pCMV-Tet3G,pTRE3G,pTRE3G-Luc, 腺病毒系统: Stratagene Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1 Strains:BJ5183,AdEasy-1 Invitrogen Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector, 腺相关病毒系统: Stratagene Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP, pCMV-MCS 逆病毒系统: RNAi Clontech Vectors:RNAi-Ready pSIREN-RetroQ, RNAi-Ready pSIREN-RetroQ-DsRed-Express RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide), 基因表达载体 Clontech Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro, pRetroX-IRES-DsRed Express Vectors:pLVTH,pSuper retro puro,pBabe puro 逆病毒包装载体 Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho 慢病毒系统: RNAi载体 Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G, pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3 Clontech Vectors:pLVX-shRNA1,pLVX-shRNA2 SBI Vectors:pSIH1-H1-CoGFP Open Biosystems Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control 基因表达载体 Clontech Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1 pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro, pLVX-Tight-Puro,pLVX-Tet-On-Advanced Invitrogen Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST Vector:pWPXL,FUGW 慢病毒包装载体 Invitrogen Vectors:pLp1,pLp2,pLp VSV G Strain:Stbl3,Stbl4 Cell biolabs Vectors:pCgpV Clontech Vector:pVSV G Second generation: Vectors:pCMV-dR8.91,pCMV-VSV-G Second generation: Vectors:pMD2.G,pSPAX2 Third generation: Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G iPS慢病毒载体 Cell biolabs Vectors:pLentG-KOSM Vectors:FUW-M2rtTA,TetO-FUW-OSKM PiggyBac转座子系统 Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector), PiggyBac 3’LTR (pMD18T vector) 信号通路报告系统: Clontech Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc, pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc pCRE-Luc,pGRE-Luc,pHSE-Luc Stratagene Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc, Promega Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19 Millipore Vectors:TOPFlash,FOPFlash,SuperTopFlash RNAi系统: Ambion Vectors:pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo, pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro Reporter plasmid:pMIR-REPORT Luciferase Promega Reproter plasmid:psiCHECK-2,pmir GLO Oligoengine Vector:pSuper-puro Other Vector:pGenesil-1 Clontech Vector:pmR mCherry SBI Vector:pmiRZip anti-microRNA 酵母表达系统: 酿酒酵母 Invitrogen Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor, Strain:INVSc1 毕赤酵母 Invitrogen Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C,pPIC9K,pPIC3.5K,pPICZ A,pPICZ B,pPICZ C, Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168, Invitrogen Vector:pPink HC,pPink LC Strain:Pichiapink-1,Pichiapink-4 Gateway系统: Invitrogen Vectors:pENTR 1A,pENTR3C,pDONR221,pcDNA6.2-GWEmGFP-miR negative, Regulatory plasmid:pLenti6/TR Strain:DB3.1 表面展示系统: 酵母表面展示 Invitrogen Vector:pYD1 Strain:EBY100 哺乳动物表面展示 Invitrogen Vector:pDisplay 噬菌体表面展示 Vector:pCantab 5e Strain:HB2151,TG1,M13KO7(in TG1) 重组蛋白乳汁表达系统: Invitrogen Vector:pBC1 绝缘子检测系统: Invivogen Vector:pWHERE 果蝇细胞表达系统: Invitrogen Vector:pAc5.1 V5-His B,pCo hygro 哺乳动物表达系统: Invitrogen Vectors:pcDNA3.1(neo)+,pcDNA3.1(neo)-,pcDNA3.1(hyg)+,pcDNA3.1(zeo)+, pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax B, pcDNA6-Myc/His B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2, pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ, pSFV1,pEF1/myc-His B,pcDNA3.1(+)/Myc HisB,pEF6/Myc-HisC, Stratagen Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B,pBK-CMV,pIRES-hrGFP-1a Promega Vectors:pCI-neo,pCI,pSI Clontech Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES hyg3, pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen), pIRES2-EGFP,pIRES puro2 Sigma Vectors:pFLAG CMV2 Invivogen Vectors:pVitro2-neo-mcs Vectors:pCAGGS 昆虫细胞表达系统: Invitrogen Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B, pBlueBacHis2 C,pVL1392,pVL1393, Strain:DH10Bac Vectors:pIZT/V5-His,pMIB v5-His B Cell:sf9,High Five MultiBac Expression System Vectors:pFBDM,pUCDM,pBADZ-His6Cre, Strains:DH10MultiBac,BW23473 Cell:sf9,High Five 荧光蛋白报告系统: Clontech Vectors:pDsRed1-C1,pDsRed2-N1,pDsRed2-C1, pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pECFP-Mito,pLEGFP-N1,pLEGFP-C1,pDsRed2-1,pEGFP-C3 FRET assay Vectors:Dkeima fluorescent protein,mAmetrine, 启动子增强子报告系统: Promega Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter, pRL-TK,pRL-CMV,pRL-SV40 NEB Vectors:pGluc-basic 原核表达系统: Novagen Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+), pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+), pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+),pET49b(+),pET50b(+), pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1 Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS, Rosetta-gami B(DE3)pLysS,BLR(DE3),BL21(DE3)gold,Rosetta Blue(DE3) Takara Vectors:pCold I,pCold TF NEB Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x Biosee Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx, pET-His,pTrc-CKS,pET-DsbA Stratagene Strain:BL21 codonplus(DE3)RIPL, Invitrogen Strain:BL21(AI) Qiagen Vector:pQE30 Strain:M15 NEB Vectors:pTWIN1,pTYB11,pMXB10,pTXB1 Strain:ER2566 Amersham Vectors:pGEX-4T-1,pGEX-5X-2,pGEX-6P-1,pGEX-2TK Invitrogen Vectors:pBADHis A,pBADHis B,pBADHis C, Other Vector:pBV220,pBluescript II SK(+) Takara 分子伴侣系统 Vector:pG-KJE8,pGro7,pKJE7,pG-Tf2,pTf16, 基因载体: Invitrogen Vector:pOG44 Invivogen Vectors:pORF-lacZ,pORF-HSV1tk 克隆载体: Promega Vectors:pUC18,pUC19,pBR322,pSP64,pSP73 柯斯载体: Stratagene Vector:SuperCos1 同源重组载体: Vector:pSIM5,pSIM6 克隆菌株: Stratagene Strains:DH5α,JM109,JM110,TOP10,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1 Phage-Resistant Cells,XL10 Gold,EZ10,DH10B,SURE, 基因: Genes: human TERT,Taq,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mous pcDNA3.2/V5-DEST ;pcDNA6/myc-His A&B&C ;pcDNA3.1(-)/myc-His A&B&C;pcDNA3.1/GS;pcDNA3.1/Zeo(+);pTracer CMV2;pBudCE4.1;pVAX1;pSecTag2 A&B&C;pSecTag A&B&C;pCDNA4/HisMax A&B&C;pcDNA3.1/V5-His A&B&C;pcDNA3.1(+)/CAT;pcDNA3.1-EGFP;pSilencer1.0;pSilencer 2.1-U6 hygro;pSilencer 3.0-H1;pSilencer 3.1-H1 hygro;pSilencer 3.1-H1 neo;pSilencer 4.1-CMV neo;pSilencer 4.1-CMV puro;pSilencer5.1-U6 Retro;pSilencer5.1-H1 Retro;pET-5a(+);pET-11a(+);pET-11b(+);

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