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电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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质粒载体菌株细胞株基因保藏中心-Biovector Science Lab可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。进入Biovector,您一定能找到所需的资源和技术服务。如有需要请联系:
Dr Xu,Senior Scientist
电邮biovector@163.com
电话l89OI268599
Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com
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标签:Biovector Inc. 质粒 载体 索取 报价 价格 优惠 售价 出售 图谱 序列 酶切位点 转化 构建 求购 购买 订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site
pTRV1 保存 病毒干扰载体 Kan kanamycin
pTRV2 保存 病毒干扰载体 Kan kanamycin
pER8-3Flag 保存 植物稳定转化 Spe Hygromycin 雌二醇诱导表达
Cam35S-GFP 保存 植物稳定转化 Kan kanamycin 与GFP融合表达
pBIN438 保存 植物稳定转化 Kan kanamycin
pCE35S-GFP 改造 植物稳定转化 Kan kanamycin 与GFP融合表达
Min 35S:: GUS 改造 瞬时作用元件分析 Kan kanamycin
pC1391-35S-YFP 改造 植物稳定转化 Kan Hygromycin 与YFP融合表达
pC1391-35S-GUS 改造 植物稳定转化 Kan Hygromycin 与GUS融合表达
pCAMBIA0380 Cambia公司 DIY Kan
pCAMBIA0390 Cambia公司 DIY Kan
pCAMBIA1381×a/b/c Cambia公司 启动子活性分析 Kan Hygromycin
pCAMBIA1391×a/b/c Cambia公司 启动子活性分析 Kan Hygromycin
pCAMBIA1300 Cambia公司 植物稳定转化 Kan Hygromycin
pCAMBIA1301 Cambia公司 植物稳定转化 Kan Hygromycin
pCAMBIA1302 Cambia公司 植物稳定转化 Kan Hygromycin
pCAMBIA1304 Cambia公司 植物稳定转化 Kan Hygromycin
pCAMBIA2200 Cambia公司 植物稳定转化 Kan kanamycin
pCAMBIA2300 Cambia公司 植物稳定转化 Kan kanamycin
pCAMBIA3301 Cambia公司 植物稳定转化 Kan Basta
pCAMBIA0380::GUS 改造 启动子活性分析 Kan 与GUS基因融合
pCAMBIA0390::GUS 改造 启动子活性分析 Kan 与GUS基因融合
pCAMBIA0390::35S-GUS 改造 启动子活性分析 Kan GUS基因前添加35S
pBI121 保存 植物稳定转化 Kan kanamycin
pBI221-GFP 保存 亚细胞定位 Amp
pRTL2-YFP 保存 亚细胞定位 Amp
pTF486 保存 亚细胞定位 Kan
pA7-YFP 保存 亚细胞定位 Amp
pSPYCE(M) 保存 BiFC Kan kanamycin
pSPYNE(R)173 保存 BiFC Kan kanamycin
pCM1307-6Myc 保存 植物稳定转化 Kan kanamycin 融合6Myc标签
pCM1307-3Flag 保存 植物稳定转化 Kan kanamycin 融合3Flag标签
pART27 保存 干扰载体 Spe kanamycin
pKANNIBAL 保存 干扰载体 Kan kanamycin
pHellsgate 8 保存 干扰载体 Spe kanamycin
pHellsgate 12 保存 干扰载体 Spe kanamycin
pME6032
pKT100
pZL507
PA01(铜绿假单胞杆菌)
YPIII(假结核鼠疫耶尔森氏菌)
pEZZ18
pSE380
pRSFduet
pYES2-EGFP
pRS316
pCDNA3.1flag
pAUR123
Staphylococcus aureus 6538
E.coli K88
E.coli K99
pAYCYDuet-1
pETDuet-1
pRSFDuet-1
pCDFDuet-1
pCold I
pCold TF
pG-KJE8
pGro7
pKJE7
pG-Tf2
pTf16
Mach1? T1 Phage-Resistant E. coli
DH5α? T1 Phage-Resistant E. coli
OmniMAX2 T1 Phage-Resistant Cells
Rosetta
Rosetta(DE3)
Rosetta(DE3) pLysS
Rosetta2 (DE3) pLysS
BL21(DE3)gold
BL21(DE3)star
Origami(DE3)
Origami2(DE3)
Rosetta-gami 2(DE3)pLysS,
Rosetta-gami B(DE3)pLysS
ArcticExpress? (DE3)RP
BL21 (DE3)gold-pRK1037
35S::GFP
35S::dsRed
35S::mCherry
pUbi::GFP
pUbi::GUS
pLH6000
pC1301s
DS1301
Bacillus subtilis 168
Bacillus subtilis 1A75
Bacillus subtilis WB600
Bacillus subtilis WB800
PMUTIN4
PSG1154
PHCMC05
PMA5 MCS
PHT304
MCF-7
K562
T2A2
B16
pASK101
Bacillus subtilis 1A75
PMA09-wapA-SP H3
PMA09-yncM-SP J3
pGFP22
pGFP315
pHT43
pWB980
pAX01
pMA5 MCS
pRS426
pCSN44
FY834
pIL253
pEGM-3ZF(+)
PUCM-T
P3-neo-loxp打靶载体
pShuttle-IRES-hrGFP-2
35S::GFP
35S::dsRed
35S::mCherry
pUbi::GFP
pUbi::GUS
pLH6000
pC1301s
DS1301
pUBIN-ZH2
pActin
ArcticExpress? (DE3)RP
Mach1? T1 Phage-Resistant E. coli
DH5α? T1 Phage-Resistant E. coli
OmniMAX2 T1 Phage-Resistant Cells
pAYCYDuet-1
pETDuet-1
pRSFDuet-1
pCDFDuet-1
pCold I
pCold TF
pG-KJE8
pGro7
pKJE7
pG-Tf2
pTf16
pME6032
pKT100
pZL507
DH5a
BL21(DE3)
PA01(铜绿假单胞杆菌)
YPIII(假结核鼠疫耶尔森氏菌)
BL21(DE3)
JM109(DE3)
枯草芽孢杆菌168
AS1.1391
产酸克雷伯士菌
W103
产气肠杆菌
副干酪乳杆菌HD1.7
米曲霉
休哈塔假丝酵母20335 HDYXHT-01
酿酒酵母W5
大肠杆菌DH10Bac
恶臭假单胞菌ATCC12633
莓实假单胞菌ACCC03879
恶臭假单胞菌ACCC10162
CICC1766
农杆菌EHA105
pMG36e
pHSG415
PYC6
P406ADH1
p2NIL
PRSFDuet-1
pet32a,pet28a, pet27b,
pklight
Pgex
pee12.4,pee6.4
PBI121
pcDNA3.0,PNZ.
Pfastbac
pVAX1-optiF
pGWB2
pENTR
杂交系统:
哺乳动物双杂交系统
Promega
Vectors:pACT,pBIND
Contorl plasmid:pACT-MyoD,pBIND-Id
Reporter plasmid:pG5 luc
Stratagene
Vectors:pCMV-BD,pCMV-AD
Contorl plasmid:pBD-p53,pBD-NF?κB,pAD-SV40T,pAD-TRAF
Reporter plasmid:pFR-luc
酵母双杂交系统
Clontech
Vectors:pGBKT7,pGADT7,
Positive contorl:pCL1,pGBKT7-53,pGADT7-T
Negative control:pGBKT7-Lam
Strain:AH109
Vector:pACT2 AD
Stratagene
Vectors:pSos,pMyr
Control plasmid:pSos MAFB,
Strain:
酵母单杂交系统
Clontech
Vector:pHis2
酵母三杂交系统
Clontech
Vector:pBridge
Strains:Y2H Gold Yeast strain,Y187
诱导系统:
蜕皮激素诱导表达系统
Invitrogen
Vectors:pIND,pVgRxR
LacSwith II哺乳动物诱导表达系统
Stratagene
Vectors:pOPRSVI,pOPI3CAT,pCMVLacI
GeneSwith 哺乳动物诱导系统
Invitrogen
Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI)
四环素诱导系统
Invitrogen
Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,
Contorl plasmid:pcDNA4/TO/Myc-His/LacZ
Regulatory plasmid:pcDNA6/TR
Clontech
Vectors:pTet-On,pTet-Off,pTRE2,pTK-hyg
pRevTet-On,pRevTet-Off,pRevTRE
pTet on advanced,pTRE Tight,pTRE Tight Luciferase
pCMV-Tet3G,pTRE3G,pTRE3G-Luc,
腺病毒系统:
Stratagene
Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1
Strains:BJ5183,AdEasy-1
Invitrogen
Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector,
腺相关病毒系统:
Stratagene
Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,
pCMV-MCS
逆病毒系统:
RNAi
Clontech
Vectors:RNAi-Ready pSIREN-RetroQ,
RNAi-Ready pSIREN-RetroQ-DsRed-Express
RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide),
基因表达载体
Clontech
Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro,
pRetroX-IRES-DsRed Express
Vectors:pLVTH,pSuper retro puro,pBabe puro
逆病毒包装载体
Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho
慢病毒系统:
RNAi载体
Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G,
pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3
Clontech
Vectors:pLVX-shRNA1,pLVX-shRNA2
SBI
Vectors:pSIH1-H1-CoGFP
Open Biosystems
Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control
基因表达载体
Clontech
Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1
pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro,
pLVX-Tight-Puro,pLVX-Tet-On-Advanced
Invitrogen
Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST
Vector:pWPXL,FUGW
慢病毒包装载体
Invitrogen
Vectors:pLp1,pLp2,pLp VSV G
Strain:Stbl3,Stbl4
Cell biolabs
Vectors:pCgpV
Clontech
Vector:pVSV G
Second generation:
Vectors:pCMV-dR8.91,pCMV-VSV-G
Second generation:
Vectors:pMD2.G,pSPAX2
Third generation:
Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G
iPS慢病毒载体
Cell biolabs
Vectors:pLentG-KOSM
Vectors:FUW-M2rtTA,TetO-FUW-OSKM
PiggyBac转座子系统
Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector),
PiggyBac 3’LTR (pMD18T vector)
信号通路报告系统:
Clontech
Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,
pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc
pCRE-Luc,pGRE-Luc,pHSE-Luc
Stratagene
Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc,
Promega
Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19
Millipore
Vectors:TOPFlash,FOPFlash,SuperTopFlash
RNAi系统:
Ambion
Vectors:pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,
pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro
Reporter plasmid:pMIR-REPORT Luciferase
Promega
Reproter plasmid:psiCHECK-2,pmir GLO
Oligoengine
Vector:pSuper-puro
Other
Vector:pGenesil-1
Clontech
Vector:pmR mCherry
SBI
Vector:pmiRZip anti-microRNA
酵母表达系统:
酿酒酵母
Invitrogen
Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor,
Strain:INVSc1
毕赤酵母
Invitrogen
Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C,pPIC9K,pPIC3.5K,pPICZ A,pPICZ B,pPICZ C,
Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168,
Invitrogen
Vector:pPink HC,pPink LC
Strain:Pichiapink-1,Pichiapink-4
Gateway系统:
Invitrogen
Vectors:pENTR 1A,pENTR3C,pDONR221,pcDNA6.2-GWEmGFP-miR negative,
Regulatory plasmid:pLenti6/TR
Strain:DB3.1
表面展示系统:
酵母表面展示
Invitrogen
Vector:pYD1
Strain:EBY100
哺乳动物表面展示
Invitrogen
Vector:pDisplay
噬菌体表面展示
Vector:pCantab 5e
Strain:HB2151,TG1,M13KO7(in TG1)
重组蛋白乳汁表达系统:
Invitrogen
Vector:pBC1
绝缘子检测系统:
Invivogen
Vector:pWHERE
果蝇细胞表达系统:
Invitrogen
Vector:pAc5.1 V5-His B,pCo hygro
哺乳动物表达系统:
Invitrogen
Vectors:pcDNA3.1(neo)+,pcDNA3.1(neo)-,pcDNA3.1(hyg)+,pcDNA3.1(zeo)+,
pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax B,
pcDNA6-Myc/His B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2,
pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ,
pSFV1,pEF1/myc-His B,pcDNA3.1(+)/Myc HisB,pEF6/Myc-HisC,
Stratagen
Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B,pBK-CMV,pIRES-hrGFP-1a
Promega
Vectors:pCI-neo,pCI,pSI
Clontech
Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES hyg3,
pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen),
pIRES2-EGFP,pIRES puro2
Sigma
Vectors:pFLAG CMV2
Invivogen
Vectors:pVitro2-neo-mcs
Vectors:pCAGGS
昆虫细胞表达系统:
Invitrogen
Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B,
pBlueBacHis2 C,pVL1392,pVL1393,
Strain:DH10Bac
Vectors:pIZT/V5-His,pMIB v5-His B
Cell:sf9,High Five
MultiBac Expression System
Vectors:pFBDM,pUCDM,pBADZ-His6Cre,
Strains:DH10MultiBac,BW23473
Cell:sf9,High Five
荧光蛋白报告系统:
Clontech
Vectors:pDsRed1-C1,pDsRed2-N1,pDsRed2-C1, pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pECFP-Mito,pLEGFP-N1,pLEGFP-C1,pDsRed2-1,pEGFP-C3
FRET assay
Vectors:Dkeima fluorescent protein,mAmetrine,
启动子增强子报告系统:
Promega
Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,
pRL-TK,pRL-CMV,pRL-SV40
NEB
Vectors:pGluc-basic
原核表达系统:
Novagen
Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+),
pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+),
pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+),pET49b(+),pET50b(+),
pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1
Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS,
Rosetta-gami B(DE3)pLysS,BLR(DE3),BL21(DE3)gold,Rosetta Blue(DE3)
Takara
Vectors:pCold I,pCold TF
NEB
Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x
Biosee
Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx,
pET-His,pTrc-CKS,pET-DsbA
Stratagene
Strain:BL21 codonplus(DE3)RIPL,
Invitrogen
Strain:BL21(AI)
Qiagen
Vector:pQE30
Strain:M15
NEB
Vectors:pTWIN1,pTYB11,pMXB10,pTXB1
Strain:ER2566
Amersham
Vectors:pGEX-4T-1,pGEX-5X-2,pGEX-6P-1,pGEX-2TK
Invitrogen
Vectors:pBADHis A,pBADHis B,pBADHis C,
Other
Vector:pBV220,pBluescript II SK(+)
Takara
分子伴侣系统
Vector:pG-KJE8,pGro7,pKJE7,pG-Tf2,pTf16,
基因载体:
Invitrogen
Vector:pOG44
Invivogen
Vectors:pORF-lacZ,pORF-HSV1tk
克隆载体:
Promega
Vectors:pUC18,pUC19,pBR322,pSP64,pSP73
柯斯载体:
Stratagene
Vector:SuperCos1
同源重组载体:
Vector:pSIM5,pSIM6
克隆菌株:
Stratagene
Strains:DH5α,JM109,JM110,TOP10,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1
Phage-Resistant Cells,XL10 Gold,EZ10,DH10B,SURE,
基因:
Genes: human TERT,Taq,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mous
pcDNA3.2/V5-DEST ;pcDNA6/myc-His A&B&C ;pcDNA3.1(-)/myc-His A&B&C;pcDNA3.1/GS;pcDNA3.1/Zeo(+);pTracer CMV2;pBudCE4.1;pVAX1;pSecTag2 A&B&C;pSecTag A&B&C;pCDNA4/HisMax A&B&C;pcDNA3.1/V5-His A&B&C;pcDNA3.1(+)/CAT;pcDNA3.1-EGFP;pSilencer1.0;pSilencer 2.1-U6 hygro;pSilencer 3.0-H1;pSilencer 3.1-H1 hygro;pSilencer 3.1-H1 neo;pSilencer 4.1-CMV neo;pSilencer 4.1-CMV puro;pSilencer5.1-U6 Retro;pSilencer5.1-H1 Retro;pET-5a(+);pET-11a(+);pET-11b(+);
FLAG-TRAF2 87-271
FLAG-TRAF2 1-271
FLAG-TRAF2 1-87
HA-GSTPi WT
FLAG-Mtraf6 FL
XP-JNKK2 WT
NF-Kb luc
JUN2-luc
HA-GSTPi 2 突变体
JNKK2 WT
HA-ASK WT
PC-DNA3.1
FLAG-TRAF6
MEKK1 FL.A
MEKK1 FL.B
MEKK1-△FLAG
C-MYC ASK FL
MKK6 DD
P38 WT A
PC-DNA3
FLAG-TRAF2 FL
TRAF2 △N
HA-Ub
TRAF6-C
FLAG-TRAF2 272-501
HA-MEKK1
HA-GSTPi
FLAG-Mtraf6-△N
PCMV-FLAG HSP27 S78/82A
PCMV-FLAG HSP27 S15A
PCMV-FLAG HSP27 (WT)
HA-HSP90a
HA-MKK3 WzG
PCDNAsFLAG GS WT
HA-HSP90b
PBS-V6
IKKb
PCMV HA
2×HA GSTPi Cys 3变
Myc-CHIP
iNOS-luc 1.3kb
U6-CHIP
PCMV-HA-TRAF6
PCMV-Myc-TRAF6
ERK WT
HA-SUMO
STAT1 Report
PET28 GSTPi WT90(克隆)
GS-PET28 BL281(表达)
PET 3C gln A
DH5a
BL21
PET28(质粒)
PET3C/B BL21(DE3)
M15 PQE/Blys
XL1
PET 43.1b(+) DNA
PET 30a-Blys Kan r
BL21(DE3)JNKK2
BL21 PET 28a JNKK2(KM)
DH5a PET 28 WT JNKK2
DH5a PET 28a
DH5a PET28 JNKK2(A)
DH5a PET28 JNKK2
XL-1 PET43b
BL21 PET28 GS
BL21 PET28a GS
BL21-Gold (DE3) 菌
BL21-Gold (DE3)-plyss
BL21-Goldonplus(DE3)-RIPL
BL21-PET 28 JNKK2 WT
PET 30a-Blys
PET30
XP-JNKK2 WT
HA-Ub
XP-HSP70
FLAG-TAK1
PCDNA 3.1-FLAG TAK1
DH5a
TRAF2 87-271
HA-GSTPi WT
Xpress GSTPi TWAL B
Xpress GSTPi TWAL A
XP-GSTPi 85-210 B
XP-GSTPi 85-210 A
XP-GSTPi 1-84 B
XP-GSTPi 1-84 A
XP-GSTPi-YST B
XP-GSTPi-YST A
PCDNA 3 B
C-mys ASK FL D
C-mys ASK FL E
TRAF2 1-271
TRAF2 272-501
JUN-luc
HA-JNK1
HA-JNK2
TRAF2 87-501
FLAG-TRAF2 1-271
HA MEKK2 FL
HA MEKK1 KM
XP-JUKK2 WT
HA-MKK3
HA-MKK6
HA-MEKK1
FLAG-TRAF2 △N
PGFP3-HSP25
HA-T6RZ
HA-T6RZC
Ub ( K63) BL21 DE3
XL-1 Blue PET 28a GFP
XP-GCK DH5a(菌)
PGFP1 HSP25
PGEFP3 HSP25(菌)
XP-JNKK2 KM
DH5a GSTPi 无tag
FLAG-TRAF2
HA-GSTPi 四突变 DH5a
FLAG-TRAF (272-501)
HA-GSTPi 三突变
GSTPi Y7
HA-TAB2 DH5a
HA-TAB1
FLAG-JNK1
DH5a
HA-ASK1
FLAG-ASK1
P53 △81
DH5a-Psuper Vector
P21-luc WAF
HA-IKBa
GST-ATF
TRAF6-DH5a
HA-TRAF2
FLAG-TRAF2
GST TRAF6 coil
GST AIF DH5a
HA-IKBa
PGEX-4T1 GST IKB
TAK1 K63A
P53 WT
GSTPi △C
GSTPi WT
B-gal DH5a
5×JUN2 luc
P38 JSF
HA-GSTPi 2突变
HA-GSTPi WT SM
MKK4
PCMV-FLAG-HSP27
PCDNA3
XP-HSP27 FL
P38 WT A
MKK4 △N
HSP70 ASK1 sense
FLAG MKK7 b1
PCMV-FLAG-HSP27
JNK-APF
HA-JNK
FLAG-MEE2.1
FLAG-MKK2 2
PCDNA3-FLAG-HSP27
GSTPi TWAL
HA-TRAF6
Myc-TRAF2
SOD-DH5a
FLAG-HSP70
FLAG-TRAF6 WT
FLAG-MKK7
Dh5a pET28 GSTpi 1-84
Dh5a pET28 GSTpi 85-210
DH5a pcDNA3 HA-GSTpi 85-210
pcDNA3 HSp27
HA-GSTpi Y7S
BL21-pET28 GSTpi Y7S
BL21-pET28 GSTpi 1-84
BL21-pET28 GSTpi 85-210
pGEX-4T-1
MEKK1
HSP27
HSP70
HA-Hsp90a
HA-HSp90b
DH5a FLAG-GST
pTRE2 HA-GSTp
DH5a pTRE2 HA-GSTp
XL-1 Blue
pET28a GSTpi WT
iNOS-luc 1.3kb
pET43b
pET28a
pET28-Hsp27
BL21
DH5a
pcMV Flag MEKK1
Flag MKK1 a1
ASK1 WT no tag
TRAF2 FL
MKK4 ΔN
p38 no tag
MEKK1 871
MKK7 a2 Flag
HA-ASK1 FL
TRAF2 1-271
MKK4 WT
XP-JNKK2 KM
ΔMEKK1 no tag
FLAG MKK7 β1
HA ASK1 ΔC
TRAF2 87-271
HA-TRAF6 WIG
XP JNKK CA
HA-MKK3
Flag TAK1 B
TRAF2 ΔN
HA-ASK1 ΔN
Flag-MKK7 β2
MEKK1 HA FL
HA MEKK1 FL2
FLAG MKK7 P1
ASK1 KN
TRAF2 272-501
FLAG TAK1 β1
HA-MKK6
HA-MEKK1 CA
FLAG MKK7 P2
XP-GCK
HA-MEKK2 FL
XP-JNKK2 WT
HA-MEKK1 KM
AP-1 LUC
HA-MEKK2 KM
FLAG-HSP70
PCDNA3
STAT1 report
p21 LUC
XP-HSP21 FL12/25
Jun2 LUC
STAT3 LUC
FLAG-TRAF6 ΔN
HA-GSTpi 3变
HA-GSTpi WT
FLAG-HSP27
GSTpi HA 4变
HA-GSTpi 47/101
GSTpi TRC no tag
GSTpi no tag2
β-gal2
β-gal1
GSTpi WT no tag
GSTpi Y7S no tag
GSTpi WT
P38 ASP
P38 β WT
P38 DN
MEKK1(CA)
MEKK1 KM
MEKK1 HA (KM)
MEKK1-f1-HA
PSRα HA-JNK1
FLAG JNK1 WT
JNKK2 KM
JNKK2 CA
JNKK2 WT
ΔMEKK1
PCDNA MKK4
SEK NL
gal 1
JNK
JNK APF
ASK1 WT
HA-ASK1
ASK1 DC-HA
ASK1-ΔN-HA
ASK KN
HA MEKK2 KM
NF-KB
HA-MEKK2 FL
MKK3
MKK6
PCDNA GSTp Y7
GSTp Δc
GSTp HA
PCDNA MKK7 α1
PCDNA MKK7 α2
PCDNA MKK7 β1
PCDNA MKK7 β2
PCDNA MKK7 P1
PCDNA MKK7 P2
AP-1 LUC 5X
P85 Δc
P110 α
P110 Δ
Akt WT
Akt(D)
P85 N
FLAG 27 S15A
FLAG 27 WT
FLAG 27 S78/82A
PCDNA3 c-Jun 73A
PCMV c-Jun 73D
xp-GCK
STAT3
STAT1
GST MU
IKB ΔN
gal
Bcl-2 Hyg
IKKβ
pcDNA FLAG-TAK1
TRAF6
HA-GST
pGFP3 hsp25
FLAG JNK-MU XL-1
FL MEKK1
FLAG △M
MKK 6E
MKK 3E
MKK 4A
MKK 4E
p38β Af
Red
ATF α
PET43a
Flag-△MEkk1
HA-T6RE
BT5183
XP-JNKK2 WT
Flag-TLR4
pcDNA-Flag-His
pcDNA-myc-His
Ikk β
Ik β
△TRAF 6
pcDNA myc
TRAF6 FL
pcDNA HA
pcMV Flag
p21-luc
GST-4变
c-myc-ASK1 FL
c-myc-ASK1 FL
Flag-JNK
Flag-JNK(mu)
MKK6 DD
HA-JNK2
Flag-JNK1
HA-JNK1
Flag-Ask1
TRAF2 87-271
TRAF2 △N
TRAF2 272-501
TRAF2 1-271
HA-Mkk3
HA-Mkk6
F-TRAF2 272-底
F-TRAF2 1-271
F-TRAF2(FL)
F-TRAF2 87-271
F-TRAF2 △N
His ubc B
His uev 1A
Flag p21
Flag-TRAF6 291-358 WC
PCMV-HA-TRAF6 WT
Flag-TRAF6 1-358 DM
Pgex GST-IkB WT
HA-TbRE
Flag-TRAF3 1-391 WC
Flag-TRAF6 1-358 WC
pGEX GST-ATF2
HA-TbREc
GST-TRAF6(coil-coil)
TRAF6 1-358
TRAF6 △292-358
His-Ubc 13
TRAF6 1-291
psuper mull 2
Ub.E.coli
NF kB luc
P-Super Hsp27 RNAi
P-Super null
HA-T6RE
Flag-TR6F6 △C.C
Flag-TR6F6 289-360
Flag-TR6F6 255-530
Flag-TR6F6 108-269
Flag-TR6F6 289-530
Flag-TR6F6 108-530
Flag-TR6F6 1-108
Flag-TR6F6 △C
His-Ucr1A kan
HA-TBRZ C
myc-△MEKK 1
myc-MEKK 1
Flag-△MEKK1
FL-Rmekk1
XP-Hsp70 △ABD
XP-Hsp70 421-642
XP-Hsp70 121-642
XP-Hsp70 △C 1-420
XP-Hsp70 1-120
XP-Hsp70 121-420
pCMV-MEK1
GSTPi RNAi
MKK6
△ IkB
Stat 3
pcDNA HA-His
Flag-TLR4
pFlag-MV2
pcDNA myc-His
mTRAF6 △N.Flag
pTRE 2 myg
PSRed N1
Flag p38(AF)
Flag p38 WT
Flag p38.(AF)
△MEKK1
HA-Mkk4
HA-MKK3
HA-MKK6
Flag-ERK WT
Flag-p38 d WT
HA-Ub
pCMV-ATF2
Flag-p38 β WT
Flag ERK2( r )
Flag-p38 δ WT
PET28a 原始
PET28a 空1
PET28a 空
PET28 GSTpi WT
PET28 GSTpi YTS
PET28 GSTpi TWAL
PET28 GSTpi WT A
PET28 JNKK2 CA
PET28 JNKK2 KM
PET28 JNKK2 WT
pcDNA3 Flag-GSTpi YTS
PET28 GSTpi 1-84 A1
PET28 GSTpi 1-84 T
PET28 GSTpi 85-210 B1
PET28 GSTpi85-210 B2
pcDNA3 HA-GST 85-210
pcDNA3 HA-GST 85-210
pcDNA3 HA-GST 1-84
PTRE2 Hyg 空
pcDNA3 Flag-TRAF2 1-87
Xpress GSTpi WT
Xpress GSTpi YTS B
Xpress GSTpi 1-84 B
Xpress GSTpi YTS A
Xpress GSTpi 85-210 B
Xpress GSTpi TWAL B
pcDNA3 HA-GST 1-84
pcDNA3 HA-GST 85-210
MKK7α2 纯
MKK7β1
MKK4
△MEKK1
GST wt
纯GSTP MV
pcDNA a
pcDNA b
2×HA GSTP Cys 4变
2×HA GSTP Cys 2变
2×HA GSTPi TWAL
Xpress GSTPi YTS
Xpress GSTPi TWAL
Xpress GSTPi WT
HA GSTP WT
Flag GSTP WT
GSTpi TRC△8 no tag
GSTpi YTS no tag
2×HA JNK WT
XP-JNKKα WT
XP-JNKKα
Xpress-JNKKα KM
Xpress-JNKKα CA
JNK-luc
iNOS-luc
NFkB-luc
Flag-TRAF6 △N
Flag-TRAF6 FL
PCMV Myc-TRAF6
HA-TRAF6
Flag-TRAFα FL
PCMV myc-TRAFα WT
Flag-TRAFα 272-501
Flag-TRAFα △N
P38 no tag
Flag-p38 β
Flag-p38 α WT
HA-ASK1 △N
HA-ASK1 FL
HA-ASK1 FL
Flag-TAK1
Flag-TLR4
Flag-TAK1(K63A)
HA-MEKK1 FL
Rat-MEKK1FL
Flag-Mkk7α1
Flag-Mkk7β1
Flag-Mkk7
Mkk6
HA-Mkk6
HA-MEKK2 FC
pcDNA3 Flag-Hsp27
pcMV Flag-Hsp27 S78/82
pcMV Flag-Hsp27 S/5A
Xpress Hsp70
Hsp70 antisense
XP-Hsp27
pGFP-Hsp25
pcDNA3 Flag-Hsp25
STAT3 report
pcDNA3
Flag-GFP
AP-1 luciferase
Flag-GFP
Flag-P21
Flag-TRAF6 1-358
Flag-TRAF6 wt
Flag-TRAF6 Δ291-358
Flag-TRAF6 ΔN
HA-CHIP
HA-hsp27 wt
HA-hsp27 Δ15A
HA-hsp27 Δ78/82A
HA-P53 wt
HA-TRAF6 wt
HA-Ub
Jun-2 luciferase
MKK7
Myc-hsp27 wt
Myc-hsp27 Δ15A
Myc-hsp27 Δ78/82A
NF-kB luciferase
P21 luciferase
PCMV-pcDNA
PET282 JNK2
PET28a JNK1
ASKc
Flag-GCS
Flag-GFP
Flag-Gs
Flag-GSTPi
Flag-Hsp27
Flag-hsp27
Flag-Hsp70
Flag-JNK1-GFP
Flag-JNK2 mut
Flag-JNK2-GFP
Flag-pcDNA3
Flag-ΔMEKK1
GFP(无Tag)
GST-red2
HA-ASK1(1-498)
HA-GlaR
HA-GS
HA-GST wt
HA-GST-2变
HA-GST-3变
HA-GST-4变
HA-GST-4变
HA-GSTPi
HA-Hsp90a
HA-hsp90β
HA-JNK1
HA-MKK3
Hsp70-antisense
JNK1-red2
JNK-APF
JNK-EGFP
MEK-EL
P21-GFP
p53
p53-Δ81
pcDNA3-2*HA-JNK2
pcDNA3-GFP
pcDNA3-GFP-JNK2
pcDNA3-GST-GFP
pcDNA3-JNK1-GFP
pcDNA3-JNK2-EGFP
pcDNA-Flag-GCS
pcDNA-GFP(单)
pcDNA-GFP(双)
pcDNA-p53-HA
PET28a
PET28-GFP
PSR-HA-JNK1
TNK1-GFP
TNK-APF
x
xp-GST
xp-GST-Y7F
xp-Hsp27
XP-hsp70
xp-hsp70 A段
xp-hsp70 wt
xp-hsp70-AC
xp-Hsp70-B
xp-hsp70-wt
xp-Hsp70-ΔC
xp-hsp70-ΔN
xp-ΔN-HSP70
pSUPER90i(amp+)
pcuv
pcDNA
HA-cdc37
HA-T6R2
HA-T6R2C
HA-HSP90a
HA-HSP90a(1-401aa)
HA-HSP90a(401-620aa)
HA-HSP90a(1-620aa)表达不好
Flag-TAK1
Psuper
HA-ubiputin
AP-Luciferase
TAB1
Flag-GFP
Flag-TAK1(1-299aa)
Flag-TAK1(301-579aa)
HA-HSP90a(1-232aa)
HA-HSP90a(233-732aa)
HA-HSP90a(402-732aa)
Akt(D)
Akt(wt)
MEK-EL
pcDNA3
BL-21-ΒET-3C-TRAIL及4种变体
BL21(DE3)
JM109(DE3)
枯草芽孢杆菌168
AS1.1391
产酸克雷伯士菌
W103
产气肠杆菌
副干酪乳杆菌HD1.7
米曲霉
休哈塔假丝酵母20335 HDYXHT-01
酿酒酵母W5
大肠杆菌DH10Bac
恶臭假单胞菌ATCC12633
莓实假单胞菌ACCC03879
恶臭假单胞菌ACCC10162
CICC1766
农杆菌EHA105
pMG36e
pHSG415
PYC6
P406ADH1
p2NIL
PRSFDuet-1
pet32a,pet28a, pet27b,
pklight
Pgex
pee12.4,pee6.4
PBI121
pcDNA3.0,PNZ.
Pfastbac
pVAX1-optiF
pGWB2
pENTR
Bacillus subtilis 1A75
PMA09-wapA-SP H3
PMA09-yncM-SP J3
pGFP22
pGFP315
pHT43
pWB980
pAX01
pMA5 MCS
pRS426
pCSN44
FY834
pIL253
pEGM-3ZF(+)
PUCM-T
P3-neo-loxp打靶载体
pShuttle-IRES-hrGFP-2
35S::GFP
35S::dsRed
35S::mCherry
pUbi::GFP
pUbi::GUS
pLH6000
pC1301s
DS1301
pUBIN-ZH2
pActin
ArcticExpress? (DE3)RP
Mach1? T1 Phage-Resistant E. coli
DH5α? T1 Phage-Resistant E. coli
OmniMAX2 T1 Phage-Resistant Cells
pAYCYDuet-1
pETDuet-1
pRSFDuet-1
pCDFDuet-1
pCold I
pCold TF
pG-KJE8
pGro7
pKJE7
pG-Tf2
pTf16
pME6032
pKT100
pZL507
DH5a
BL21(DE3)
PA01(铜绿假单胞杆菌)
YPIII(假结核鼠疫耶尔森氏菌)
BL21(DE3)
JM109(DE3)
枯草芽孢杆菌168
AS1.1391
产酸克雷伯士菌
W103
产气肠杆菌
副干酪乳杆菌HD1.7
米曲霉
休哈塔假丝酵母20335 HDYXHT-01
酿酒酵母W5
大肠杆菌DH10Bac
恶臭假单胞菌ATCC12633
莓实假单胞菌ACCC03879
恶臭假单胞菌ACCC10162
CICC1766
农杆菌EHA105
pMG36e
pHSG415
PYC6
P406ADH1
p2NIL
PRSFDuet-1
pet32a,pet28a, pet27b,
pklight
Pgex
pee12.4,pee6.4
PBI121
pcDNA3.0,PNZ.
Pfastbac
pVAX1-optiF
pGWB2
pENTR
杂交系统:
哺乳动物双杂交系统
Promega
Vectors:pACT,pBIND
Contorl plasmid:pACT-MyoD,pBIND-Id
Reporter plasmid:pG5 luc
Stratagene
Vectors:pCMV-BD,pCMV-AD
Contorl plasmid:pBD-p53,pBD-NF?κB,pAD-SV40T,pAD-TRAF
Reporter plasmid:pFR-luc
酵母双杂交系统
Clontech
Vectors:pGBKT7,pGADT7,
Positive contorl:pCL1,pGBKT7-53,pGADT7-T
Negative control:pGBKT7-Lam
Strain:AH109
Vector:pACT2 AD
Stratagene
Vectors:pSos,pMyr
Control plasmid:pSos MAFB,
Strain:
酵母单杂交系统
Clontech
Vector:pHis2
酵母三杂交系统
Clontech
Vector:pBridge
Strains:Y2H Gold Yeast strain,Y187
诱导系统:
蜕皮激素诱导表达系统
Invitrogen
Vectors:pIND,pVgRxR
LacSwith II哺乳动物诱导表达系统
Stratagene
Vectors:pOPRSVI,pOPI3CAT,pCMVLacI
GeneSwith 哺乳动物诱导系统
Invitrogen
Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI)
四环素诱导系统
Invitrogen
Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,
Contorl plasmid:pcDNA4/TO/Myc-His/LacZ
Regulatory plasmid:pcDNA6/TR
Clontech
Vectors:pTet-On,pTet-Off,pTRE2,pTK-hyg
pRevTet-On,pRevTet-Off,pRevTRE
pTet on advanced,pTRE Tight,pTRE Tight Luciferase
pCMV-Tet3G,pTRE3G,pTRE3G-Luc,
腺病毒系统:
Stratagene
Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1
Strains:BJ5183,AdEasy-1
Invitrogen
Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector,
腺相关病毒系统:
Stratagene
Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,
pCMV-MCS
逆病毒系统:
RNAi
Clontech
Vectors:RNAi-Ready pSIREN-RetroQ,
RNAi-Ready pSIREN-RetroQ-DsRed-Express
RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide),
基因表达载体
Clontech
Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro,
pRetroX-IRES-DsRed Express
Vectors:pLVTH,pSuper retro puro,pBabe puro
逆病毒包装载体
Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho
慢病毒系统:
RNAi载体
Vectors:pSicoR,pSicoR pGK puro,pLentilox 3.7,pLKO.1 puro,pLKO.3G,
pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3
Clontech
Vectors:pLVX-shRNA1,pLVX-shRNA2
SBI
Vectors:pSIH1-H1-CoGFP
Open Biosystems
Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control
基因表达载体
Clontech
Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1
pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro,
pLVX-Tight-Puro,pLVX-Tet-On-Advanced
Invitrogen
Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST
Vector:pWPXL,FUGW
慢病毒包装载体
Invitrogen
Vectors:pLp1,pLp2,pLp VSV G
Strain:Stbl3,Stbl4
Cell biolabs
Vectors:pCgpV
Clontech
Vector:pVSV G
Second generation:
Vectors:pCMV-dR8.91,pCMV-VSV-G
Second generation:
Vectors:pMD2.G,pSPAX2
Third generation:
Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G
iPS慢病毒载体
Cell biolabs
Vectors:pLentG-KOSM
Vectors:FUW-M2rtTA,TetO-FUW-OSKM
PiggyBac转座子系统
Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector),
PiggyBac 3’LTR (pMD18T vector)
信号通路报告系统:
Clontech
Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,
pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc
pCRE-Luc,pGRE-Luc,pHSE-Luc
Stratagene
Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc,
Promega
Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19
Millipore
Vectors:TOPFlash,FOPFlash,SuperTopFlash
RNAi系统:
Ambion
Vectors:pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,
pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro
Reporter plasmid:pMIR-REPORT Luciferase
Promega
Reproter plasmid:psiCHECK-2,pmir GLO
Oligoengine
Vector:pSuper-puro
Other
Vector:pGenesil-1
Clontech
Vector:pmR mCherry
SBI
Vector:pmiRZip anti-microRNA
酵母表达系统:
酿酒酵母
Invitrogen
Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor,
Strain:INVSc1
毕赤酵母
Invitrogen
Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C,pPIC9K,pPIC3.5K,pPICZ A,pPICZ B,pPICZ C,
Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168,
Invitrogen
Vector:pPink HC,pPink LC
Strain:Pichiapink-1,Pichiapink-4
Gateway系统:
Invitrogen
Vectors:pENTR 1A,pENTR3C,pDONR221,pcDNA6.2-GWEmGFP-miR negative,
Regulatory plasmid:pLenti6/TR
Strain:DB3.1
表面展示系统:
酵母表面展示
Invitrogen
Vector:pYD1
Strain:EBY100
哺乳动物表面展示
Invitrogen
Vector:pDisplay
噬菌体表面展示
Vector:pCantab 5e
Strain:HB2151,TG1,M13KO7(in TG1)
重组蛋白乳汁表达系统:
Invitrogen
Vector:pBC1
绝缘子检测系统:
Invivogen
Vector:pWHERE
果蝇细胞表达系统:
Invitrogen
Vector:pAc5.1 V5-His B,pCo hygro
哺乳动物表达系统:
Invitrogen
Vectors:pcDNA3.1(neo)+,pcDNA3.1(neo)-,pcDNA3.1(hyg)+,pcDNA3.1(zeo)+,
pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax B,
pcDNA6-Myc/His B,pSecTag2 A,pVAX1,pBudCE4.1,pTracer CMV2,
pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ,
pSFV1,pEF1/myc-His B,pcDNA3.1(+)/Myc HisB,pEF6/Myc-HisC,
Stratagen
Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B,pBK-CMV,pIRES-hrGFP-1a
Promega
Vectors:pCI-neo,pCI,pSI
Clontech
Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES hyg3,
pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen),
pIRES2-EGFP,pIRES puro2
Sigma
Vectors:pFLAG CMV2
Invivogen
Vectors:pVitro2-neo-mcs
Vectors:pCAGGS
昆虫细胞表达系统:
Invitrogen
Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B,
pBlueBacHis2 C,pVL1392,pVL1393,
Strain:DH10Bac
Vectors:pIZT/V5-His,pMIB v5-His B
Cell:sf9,High Five
MultiBac Expression System
Vectors:pFBDM,pUCDM,pBADZ-His6Cre,
Strains:DH10MultiBac,BW23473
Cell:sf9,High Five
荧光蛋白报告系统:
Clontech
Vectors:pDsRed1-C1,pDsRed2-N1,pDsRed2-C1, pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pECFP-Mito,pLEGFP-N1,pLEGFP-C1,pDsRed2-1,pEGFP-C3
FRET assay
Vectors:Dkeima fluorescent protein,mAmetrine,
启动子增强子报告系统:
Promega
Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,
pRL-TK,pRL-CMV,pRL-SV40
NEB
Vectors:pGluc-basic
原核表达系统:
Novagen
Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+),
pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+),
pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+),pET49b(+),pET50b(+),
pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1
Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS,
Rosetta-gami B(DE3)pLysS,BLR(DE3),BL21(DE3)gold,Rosetta Blue(DE3)
Takara
Vectors:pCold I,pCold TF
NEB
Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x
Biosee
Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx,
pET-His,pTrc-CKS,pET-DsbA
Stratagene
Strain:BL21 codonplus(DE3)RIPL,
Invitrogen
Strain:BL21(AI)
Qiagen
Vector:pQE30
Strain:M15
NEB
Vectors:pTWIN1,pTYB11,pMXB10,pTXB1
Strain:ER2566
Amersham
Vectors:pGEX-4T-1,pGEX-5X-2,pGEX-6P-1,pGEX-2TK
Invitrogen
Vectors:pBADHis A,pBADHis B,pBADHis C,
Other
Vector:pBV220,pBluescript II SK(+)
Takara
分子伴侣系统
Vector:pG-KJE8,pGro7,pKJE7,pG-Tf2,pTf16,
基因载体:
Invitrogen
Vector:pOG44
Invivogen
Vectors:pORF-lacZ,pORF-HSV1tk
克隆载体:
Promega
Vectors:pUC18,pUC19,pBR322,pSP64,pSP73
柯斯载体:
Stratagene
Vector:SuperCos1
同源重组载体:
Vector:pSIM5,pSIM6
克隆菌株:
Stratagene
Strains:DH5α,JM109,JM110,TOP10,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1
Phage-Resistant Cells,XL10 Gold,EZ10,DH10B,SURE,
基因:
Genes: human TERT,Taq,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mous
pcDNA3.2/V5-DEST ;pcDNA6/myc-His A&B&C ;pcDNA3.1(-)/myc-His A&B&C;pcDNA3.1/GS;pcDNA3.1/Zeo(+);pTracer CMV2;pBudCE4.1;pVAX1;pSecTag2 A&B&C;pSecTag A&B&C;pCDNA4/HisMax A&B&C;pcDNA3.1/V5-His A&B&C;pcDNA3.1(+)/CAT;pcDNA3.1-EGFP;pSilencer1.0;pSilencer 2.1-U6 hygro;pSilencer 3.0-H1;pSilencer 3.1-H1 hygro;pSilencer 3.1-H1 neo;pSilencer 4.1-CMV neo;pSilencer 4.1-CMV puro;pSilencer5.1-U6 Retro;pSilencer5.1-H1 Retro;pET-5a(+);pET-11a(+);pET-11b(+);
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