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Taq,Pfu高表达载体菌株KOD, T4连接酶高表达质粒载体,高产基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反转录酶MMLV,Klentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000质粒
荧光蛋白表达载体荧光定位质粒报告基因载体pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 启动子增强子报告载体(Promega,带有Luc荧光报告基因): pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信号通路报告载体(Clontech): pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP, pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc 信号通路报告载体(Promega): pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信号通路报告载体(Stratagene): p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc, pFA2-Elk1,pFC-MEKK,pFR-luc
pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 转座
子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1,
杂交系统:
哺乳动物双杂交系统
Promega
Vectors:pACT,pBIND
Contorl plasmid:pACT-MyoD,pBIND-Id
Reporter plasmid:pG5 luc
Stratagene
Vectors:pCMV-BD,pCMV-AD
Contorl plasmid:pBD-p53,pBD-NF−κB,pAD-SV40T,pAD-TRAF
Reporter plasmid:pFR-luc
酵母双杂交系统
Clontech
Vectors:pGBKT7,pGADT7,
Positive contorl:pCL1,pGBKT7-53,pGADT7-T
Negative control:pGBKT7-Lam
Strain:AH109
Vector:pACT2 AD
Stratagene
Vectors:pSos,pMyr
Control plasmid:pSos MAFB,
酵母单杂交系统
Clontech
Vector:pHis2,pHISi,pHISi-1,pLacZi,pGAD424 ,pGAD53m,p53BLUE,p53HIS
酵母三杂交系统
Clontech
Vector:pBridge
Strains:Y2H Gold Yeast strain,Y187
诱导系统:
蜕皮激素诱导表达系统
Invitrogen
Vectors:pIND,pVgRxR
LacSwith II哺乳动物诱导表达系统
Stratagene
Vectors:pOPRSVI,pOPI3CAT,pCMVLacI
GeneSwith 哺乳动物诱导系统
Invitrogen
Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI)
四环素诱导系统
Invitrogen
Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,
pcDNA4/TO/Myc-His C,
Contorl plasmid:pcDNA4/TO/Myc-His/LacZ
Regulatory plasmid:pcDNA6/TR
Clontech
Vectors:pTet-On,pTet-Off,pTRE2,pTRE2 hyg(mcs区含有插入片段),pTK-hyg
pRevTet-On,pRevTet-Off,pRevTRE
pTet on advanced,pTRE Tight,pTRE Tight Luciferase
pCMV-Tet3G,pTRE3G,pTRE3G-Luc,
Takara分子伴侣系统:
Vectors:pTf16,pG-Tf2,pG-KJE8,pKJE7
腺病毒系统:
Stratagene
Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1,pShuttle-IRES-hrGFP-2,pAdTrack-CMV/beta-gal
Strains:BJ5183,AdEasy-1
Invitrogen
Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector,
microbix公司AdMax腺病毒:
pEarleygate103,pDC315
腺相关病毒系统:
Stratagene
Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS, pAAV-EGFP,pAAV-U6-ires-hrGFP,pAAV-Rc1型,pAAV-Rc8型,pAAV-Rc9型,
逆病毒系统:
RNAi
Clontech
Vectors:RNAi-Ready pSIREN-RetroQ,
RNAi-Ready pSIREN-RetroQ-DsRed-Express
RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide),
Ambion
Vectors:pSilencer5.1-U6-Retro,pSilencer5.1-H1-Retro
基因表达载体
Clontech
Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro,pMSCVneo,pMSCV-EGFP,pLXSN
pRetroX-IRES-DsRed Express,
Addgene
Vectors:,pSuper retro puro,pBabe puro
逆病毒包装载体
Stratagene
Vectors:pVPack-GP,pCMV VSV G
Addgene
Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho
慢病毒系统:
RNAi载体
Addgene
Vectors:pSicoR,pSicoR pGK puro,pLL 3.7,pLKO.1 - TRC Cloning,pLKO.3G,
pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3,pLVTH,pLVTHM
Clontech
Vectors:pLVX-shRNA1,pLVX-shRNA2
SBI
Vectors:pSIH1-H1-CoGFP,pPS-rtTA3R-Puro,pPS-rtTA3R-RFP,pPS-TRE-GFP-miR,pPS-TRE-RFP-miR
Open Biosystems
Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control
基因表达载体
Clontech
Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1
pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro,
pLVX-Tight-Puro,pLVX-Tet-On-Advanced
Invitrogen
Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST,pLenti6-UbC-V5-DEST
Addgene
Vector:pWPXL,FUGW,pLVTH,pLVTHM
慢病毒包装载体
Invitrogen
Vectors:pLp1,pLp2,pLp /VSVG
Strain:Stbl3,Stbl4
Cell biolabs
Vectors:pCgpV
Clontech
Vector:pVSV G
Addgene
Second generation:
Vectors:pCMV-dR8.91,pCMV-VSV-G
Second generation:
Vectors:pMD2.G,pSPAX2
Third generation:
Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G(pMDLg/pRRE + pRSV-Rev + pMD2.G)
iPS慢病毒载体
Cell biolabs
Vectors:pLentG-KOSM
Addgene
Vectors:FUW-M2rtTA,TetO-FUW-OSKM
PiggyBac转座子系统
Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector),
PiggyBac 3’LTR (pMD18T vector)
信号通路报告系统:
Clontech
Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc,
pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc
pCRE-Luc,pGRE-Luc,pHSE-Luc
Stratagene
Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFB-luc
Promega
Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19
Millipore
Vectors:TOPFlash,FOPFlash,SuperTopFlash
国产:
pZRT1-Luc
RNAi系统:
Ambion
Vectors: pSilencer 1.0-U6(pBS/U6),pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro,
Reporter plasmid:pMIR-REPORT Luciferase
Promega
Reproter plasmid:psiCHECK-2,pmir GLO
Oligoengine
Vector:pSuper-puro,pSUPER retro Puro
Other
Vector:pGenesil-1, pucrnai,
Clontech
Vector:pmR mCherry
SBI
Vector:pmiRZip anti-microRNA
酵母表达系统:
酿酒酵母
Invitrogen
Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor,pYES3/CT
Strain:INVSc1
毕赤酵母
Invitrogen
Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C pPIC9K,pPIC3.5K pPICZ A,pPICZ B,pPICZ C,
Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168
Invitrogen
Vector:pPink HC,pPink LC
Strain:Pichiapink-1,Pichiapink-4
Gateway系统:
Invitrogen
Vectors:pENTR 1A,pENTR3C,pDONR221, pcDNA6.2-GWEmGFP-miR negative,
Regulatory plasmid:pLenti6/TR
Strain:DB3.1
表面展示系统:
酵母表面展示
Invitrogen
Vector:pYD1
Strain:EBY100
哺乳动物表面展示
Invitrogen
Vector:pDisplay
噬菌体表面展示
Vector:pCantab 5e
Strain:HB2151,TG1,M13KO7(in TG1)
重组蛋白乳汁表达系统:
Invitrogen
Vector:pBC1
分泌型真核表达载体:
Invitrogen
Vector:PsecTag2A,PsecTag2C
绝缘子检测系统:
Invivogen
Vector:pWHERE
果蝇细胞表达系统:
Invitrogen
Vector:pAc5.1 V5-His B,pCo hygro
哺乳动物表达系统:
Invitrogen
Vectors:pcDNA3.1(+)neo,pcDNA3.1(-)neo ,pcDNA3.1(+)hyg,pcDNA3.1(+)zeo,
pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax A,pcDNA4/HisMax B,pcDNA4/HisMax C,pcDNA4/HisMax lacZ,pcDNA6-Myc/His B,pSecTag2 A,pVAX1,PVAX1-lacZ ,pBudCE4.1,pTracer CMV2, pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ,pSFV1,pEF1/myc-His B,pCDNA3.0,pCDNA3.0/3C-myc,pCDNA3.0/3flag,pCDNA3.0/3HA,pCDNA3.0/5flag,pCDNA3.0/5HA,pCDNA3.0/beta-gal,pCDNA3.0/EGFP/C,pCDNA3.0/EGFP/N,pcDNA3.1D/v5-His,pBAD/His A,pEF6/Myc-HisB, pEF6/Myc-HisC
Stratagen
Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B, pBK-CMV,pIRES-hrGFP-1a,pIRES-hrGFP-2a, pSG5
Promega
Vectors:pCI-neo,pCI,pSI
Clontech
Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES neo2,pIRES hyg3,
pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen),
pIRES2-EGFP,pIRES2-DsRed-Express ,pIRES puro2,
Sigma
Vectors:pFLAG CMV2,pFlag-CMV1
Invivogen
Vectors:pVitro2-neo-mcs
Addgene
Vectors:pCAGGS
Invivogen
Vector:pFUSE-CHIg-hG1(modified)
昆虫细胞表达系统:
Invitrogen
Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B,
pBlueBacHis2 C,pVL1392,pVL1393, Strain:DH10Bac
Vectors:pIZT/V5-His,pMIB v5-His B
Cell:sf9 , High Five
MultiBac Expression System
Vectors:pFBDM,pUCDM,pBADZ-His6Cre,
Strains:DH10MultiBac,BW23473
Cell:sf9
植物表达载体:
pCAMBIA 1300,pCAMBIA 1301,pCAMBIA 1305,pcambia 1302, pBI 121
荧光蛋白报告系统:
Clontech
Vectors:pDsRed1-N1,pDsRed1-C1,pDsRed2-N1,pDsRed2-C1pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N2,pEGFP-C2,pEGFP-N3,pEGFP-C3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pLEGFP-N1,pLEGFP-C1, pGFPuv, pECFP-Mito,pDsRed2-1,pPAmCherry-Tubulin
FRET assay
Vectors:Dkeima fluorescent protein,mAmetrine,
启动子增强子报告系统:
Promega
Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,
pRL-TK,pRL-CMV,pRL-SV40
NEB
Vectors:pGluc-basic
原核表达系统:
Novagen
Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+),
pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+),
pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+), pET49b(+),pET50b(+),
pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1, pET43.1a+
Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS,
Rosetta-gami B(DE3)pLysS,BLR(DE3), BL21(DE3)gold,Rosetta Blue(DE3)
Takara
Vectors:pCold I,pCold TF
NEB
Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x
Biosee
Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx,
pET-His,pTrc-CKS,pET-DsbA
Stratagene
Vector:pBluescript II KS(+),pBluescript II KS(+)-EGFP,pBluescript II sk(+),
Strain:BL21 codonplus(DE3)RIPL
Invitrogen
Strain:BL21(AI)
Qiagen
Vector:pQE 30,PQE 31,PQE 60,PQE 70,PQE 9,
Strain:M15
NEB
Vectors:pTWIN1,pTYB11,pMXB10,pTXB1
Strain:ER2566
Amersham
Vectors:pGEX-4T-1,PGEX-5X-1,pGEX-6P-1,pGEX-2TK,pGEX-5X-2
Invitrogen
Vectors:pBADHis A,pBADHis B,pBADHis C,
Lucigen
Strain:C41(DE3),C43(DE3)
其他:
Vectors:PHB201,pALTER-1
温控表达系统:
PBV221
基因载体:
Invitrogen
Vector:pOG44
Invivogen
Vectors:pORF-lacZ,pORF-HSV1tk
克隆载体:
Promega
Vectors:pUC18,pUC19,pBR322,pSP64,pSP73,pCR-Blunt(环状),pUCm-T(环状),pBS-T(环状),PBS-T S (环状),pSV-beta-gal,pRS315-Flag,pRS315-myc,
柯斯载体:
Stratagene
Vector:SuperCos1
同源重组载体:
Vector:pSIM5,pSIM6
其他:
pSAL1,pspT18,pspT18-Neo,pspT19,pspT19-Neo,
克隆菌株:
Stratagene
Strains:DH5α,JM109,JM110,TOP10,BL21,GM2163,BLT5403,BLT5615,XL1-Blue,XL1-Blue MRF’,XLOLR,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1 Phage-Resistant Cells,ExAssist interference-resistant helper phage, R408 Interference Resistant Helper Phage, XL10 Gold,EZ10
基因:
Genes: human TERT,Tag,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mouse IL18,Cre酶,rat 91ES8R(X80155),human HIF1A(NM_001530),human GADD45A,human GADD45B,human GADD45G,human Hegf(AAA60744),human Vegf165(AF486837),human FGF5(NM_033143),human P53(NM_000546),human VEGF121(AF214570),human CXCR4,human HSP40,human UBE2I,human FKBP38,human FKBP12,human IP-10,human arr2,human arr1,human HVEM(NM_003820.2),human PPP1CC(NM_002710),human RICTOR(NM_152756),human SOCS2(NM_003877),human FADD(NM_003824.3),human ID1(NM_002165.2),human STAT3(NM_139276),human NOTCH2(NM_024408.2),human KRT19(NM_002276),human KLK3(NM_001648),human CEACAM5(NM_004363),human CGB(NM_000737),human GRWD1(NM_031485),human RELT(NM_032871),human RELT(NM_152222),human AFP(NM_001134),human ASB9(NM_001031739),human ASB9(NM_024087),human ASB9(NM_001168530),human ASB9(NM_001168531),human KLF17(NM_173484),human RELT(NM_032871),human RELT(NM_152222),human KLK3(NM_001648),human EMA6C (NM_030913.3),human DUSP8 (NM_004420.2),human SAPS2 (NM_014678.3),human RPS6,human PPP6R2(NM_014678),human beclin1,human ATG5,human ATG12,human SLC10A1,human SLC10A2 ,human RIPK3(NM_006871.3),human RIPK1,pET-24a-SacB,human IER5(NM_016545),
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