Product Data Sheet
|
Order ID |
Name |
Description |
|
Biovector-01128569 |
pCMV-KSRP |
pCMV-KSRP,in E.coli,300uL,AmpR. Storage:4℃ |
Vector Information
ORF Sequence Information
atgtcg gactacagca cgggaggacc cccgcccggg ccgccgccgc
121 ccgccggcgg gggcggggga gccggaggcg ccgggggagg ccctccgccg ggcccgccag
181 gcgcggggga ccggggcggc ggcggtcccg gcggcggcgg cccgggcggg gggtcggccg
241 ggggcccctc tcagccaccc ggcggaggcg gcccgggaat ccgcaaggac gctttcgccg
301 acgccgtgca gcgggcccgc cagattgcag ccaaaattgg aggcgatgct gccacgacag
361 tgaataacag cactcctgat tttggttttg ggggccaaaa gagacagttg gaagatggag
421 atcaaccgga gagcaagaag ctggcttccc agggagactc aatcagttct caacttggac
481 ccatccatcc tcccccaagg acttcaatga cagaagagta cagggtccca gacggcatgg
541 tgggcctgat cattggcaga ggaggtgaac aaattaacaa aatccaacag gattcaggct
601 gcaaagtaca gatttctcca gacagcggtg gcctacccga gcgcagtgtg tccttgacag
661 gagccccaga atctgtccag aaagccaaga tgatgctgga tgacattgtg tctcggggtc
721 gtgggggccc cccaggacag ttccacgaca acgccaacgg gggccagaac ggcaccgtgc
781 aggagatcat gatccccgcg ggcaaggccg gcctggtcat tggcaagggc ggggagacca
841 ttaagcagct gcaggaacgc gctggagtga agatgatctt aattcaggac ggatctcaga
901 atacgaatgt ggacaaacct ctccgcatca ttggggatcc ttacaaagtg cagcaagcct
961 gtgagatggt gatggacatc ctccgggaac gtgaccaagg cggctttggg gaccggaatg
1021 agtacggatc tcggattggc ggaggcatcg atgtgccagt gcccaggcat tctgttggcg
1081 tggtcattgg ccggagtgga gagatgatca agaagatcca gaatgatgct ggcgtgcgga
1141 tacagttcaa gcaagatgac gggacagggc ccgagaagat tgctcatata atggggcccc
1201 cagacaggtg cgagcacgca gcccggatca tcaacgacct cctccagagc ctcaggagtg
1261 gtcccccagg tcctccaggg ggtccaggca tgcccccggg gggccgaggc cgaggaagag
1321 gccaaggcaa ttggggtccc cctggcgggg agatgacctt ctccatcccc actcacaagt
1381 gtgggctggt catcggccga ggtggcgaga atgtgaaagc cataaaccag cagacgggag
1441 ccttcgtaga gatctcccgg cagctgccac ccaacgggga ccccaacttc aagttgttca
1501 tcatccgggg ttcaccccag cagattgacc acgccaagca gcttatcgag gaaaagatcg
1561 agggtcctct ctgcccagtt ggaccaggcc caggtggccc aggccctgct ggcccaatgg
1621 ggcccttcaa tcctgggccc ttcaaccagg ggccacccgg ggctccccca catgccgggg
1681 ggccccctcc tcaccagtac ccaccccagg gctggggcaa tacctacccc cagtggcagc
1741 cgcctgctcc tcatgaccca agcaaagcag ctgcagcggc cgcggacccc aacgccgcgt
1801 gggccgccta ctactcacac tactaccagc agcccccggg ccccgtcccc ggccccgcac
1861 cggcccctgc ggccccaccg gctcagggtg agccccctca gcccccaccc accggccagt
1921 cggactacac taaggcctgg gaagagtatt acaaaaagat cggccagcag ccccagcagc
1981 ccggagcgcc cccacagcag gactacacga aggcttggga ggagtactac aagaagcaag
2041 cgcaagtggc caccggaggg ggtccaggag ctcccccagg cttcctggcc ccccccacgg
2101 tgttccagcc cctcctctct tccgccctgg ctcagctccc gcggccccgc ccgtcccccc
2161 tcccaggact ggtctgttgt cttttcatct gttcaagagg agattga
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
