粟酒裂殖酵母表达载体pESP-2，pESP2

出品公司:	Stratagene
质粒类型:	粟酒裂殖酵母表达载体
启动子:	nmt1
载体标签:	N-GST,N-Flag
酶识别位点:	thrombin,EK
筛选标记:	LEU2-d
载体抗性:	Ampicillin

产品编号	产品名称	规格	价格
Biovector2237	pESP-2	5ug质粒	¥3920.00

The ESP® yeast protein expression and purification system uses the yeast Schizosaccharomyces pombe as the expression host and the glutathione S-transferase (GST) peptide as the protein purification tag. This system provides an easy alternative to protein production in E. coli. Proteins expressed in E. coli may lack proper biological function and antigenicity because of the absence of eukaryotic posttranslational modifications. S. pombe is a single-cell eukaryotic organism with properties similar to higher eukaryotic organisms. These properties, such as chromosome structure and function, cell-cycle control, RNA splicing and codon usage, make S. pombe ideal for the production of eukaryotic proteins. Also, eukaryotic proteins expressed in S. pombe are more likely to be folded properly, which improves the specific activity and can eliminate protein insolubility problems found in E. coli expression systems.

Location of Feature

ARS1 origin 65C1270
ampicillin resistance (bla) ORF 1401C2258
pUC origin 2409C3076
yeast LEU2-d (promoter mutant) ORF 3909C5006
S. pombe nmt1 promoter 5945C7118
GST affinity tag 7121C7771
thrombin target 7793C7860
FLAG tag 7781C7798
EK target 7799C7822
multiple cloning site 7808C7822
S. pombe nmt1 terminator 7877C8871
f1 origin 9086C9392

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