粟酒裂殖酵母表达载体pESP-1，pESP1

出品公司:	Stratagene
质粒类型:	粟酒裂殖酵母表达载体
启动子:	nmt1
载体标签:	N-GST,N-Flag
酶识别位点:	thrombin,EK
筛选标记:	LEU2-d
载体抗性:	Ampicillin

产品编号	产品名称	规格	价格
VT2236	pESP-1	5ug质粒	¥1500.00

The ESP® yeast protein expression and purification system uses the yeast Schizosaccharomyces pombe as the expression host and the glutathione S-transferase (GST) peptide as the protein purification tag. This system provides an easy alternative to protein production in E. coli. Proteins expressed in E. coli may lack proper biological function and antigenicity because of the absence of eukaryotic posttranslational modifications. S. pombe is a single-cell eukaryotic organism with properties similar to higher eukaryotic organisms. These properties, such as chromosome structure and function, cell-cycle control, RNA splicing and codon usage, make S. pombe ideal for the production of eukaryotic proteins. Also, eukaryotic proteins expressed in S. pombe are more likely to be folded properly, which improves the specific activity and can eliminate protein insolubility problems found in E. coli expression systems.

Location of Features

ARS1 origin 65C1270
ampicillin resistance (bla) ORF 1401C2258
pUC origin 2409C3076
yeast LEU2-d (promoter mutant) ORF 5464C6555
S. pombe nmt1 promoter 7001C8165
GST affinity tag 8168C8818
thrombin target 8870C8881
FLAG tag 8828C8845
EK target 8846C8869
multiple cloning site 8855C8869
S. pombe nmt1 terminator 8885C9879
f1 origin 10091C10397

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