pRB473金黄色葡萄球菌穿梭表达载体Staphylococcus aureus

价　　格：¥7930
货　　号：3497324
产　　地：北京

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Product Data Sheet

Order ID	Name	Description
Biovector168929	pRB473	pRB473 vector. in E.coli, 300uL,AmpR. Storage:4℃

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.

2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6. Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.