Product Data Sheet
Order ID | Name | Description |
Biovector-01128569 | pUTgfp, pUTmini-Tn5-GFP | pUTgfp, pUTmini-Tn5-GFP, in E.coli, AmpR. Storage:4℃ |
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
质粒载体菌株细胞株基因保藏中心Biovector Science Lab，Inc可提供近万种质粒载体、菌株、基因和细胞株，并可提供实验技术外包服务，如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi、蛋白质组学研究、色谱/质谱鉴定等服务。做为Addgene质粒载体基因菌株储藏中心分库及唯一办事处，Biovector Science Lab可为您提供便捷一站式的Addgene产品服务。进入Biovector,您一定能从这找到所需要的资源和技术服务。
如有需要请联系：
Dr Guo,Senior Scientist
手机189Ol268599
电邮biovector@163.com
Biovector实验室质粒载体菌株基因细胞株列表网址http://biovector.blog.163.com
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