Product Data
Sheet
|
Order ID
|
Name
|
Description
|
|
Biovector113372
|
pComb3x
|
pComb3x,AmpR. 300uL, in E.coli. Storage:4℃
|
Recovery
1.Obtain
an LB agar plate with the appropriate antibiotic.
2.Using
a sterile pipette tip, touch the bacteria growing within the punctured area of
the stab culture. (A sterilized wire loop or sterile toothpick can be used in
place of a sterile pipette tip.)
3.Run
this tip lightly over a section of the plate, as shown in the figure, to create
streak #1.
4.Using
another sterile pipette tip, pass through streak #1 and spread the bacteria
over a second section of the plate, to create streak #2.
5.Using
a third sterile pipette tip, pass through streak #2 and spread the bacteria
over the last section of the plate, to create streak #3.
6.Grow
overnight in a 37 o C incubator (unless a different growth
temperature is indicated on the plasmid datasheet).
7.In
the morning, single colonies should be visible. If the bacterial growth is too
dense, re-streak onto a new agar plate to obtain single colonies.
质粒载体菌株细胞株基因保藏中心Biovector Science Lab，Inc可提供近万种质粒载体、菌株、基因和细胞株，并可提供实验技术外包服务，如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi、蛋白质组学研究、色谱/质谱鉴定等服务。做为Addgene质粒载体基因菌株储藏中心分库及唯一办事处，Biovector Science Lab可为您提供便捷一站式的Addgene产品服务。进入Biovector,您一定能从这找到所需要的资源和技术服务。
如有需要请联系：
Dr Guo,Senior Scientist
手机189Ol268599
电邮biovector@163.com
Biovector实验室质粒载体菌株基因细胞株列表网址http://biovector.blog.163.com
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