pQE-TriSystem原核表达载体

载体基本信息 出品公司: Qiagen 载体名称: pQE-TriSystem 质粒类型: 原核表达载体；昆虫表达载体；哺乳细胞表达载体 高拷贝/低拷贝: 高拷贝 启动子: P10启动子 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5766 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: 8xHis 载体抗性: 氨苄青霉素 筛选标记: -- 备注: 高水平蛋白表达 产品目录号: -- 稳定性: 瞬时表达 组成型: 非组成型 病毒/非病毒: 非病毒 载体质粒图谱和多克隆位点信息 pQE-TriSystem载体图谱 pQE-TriSystem多克隆位点1 pQE-TriSystem多克隆位点2 pQE-TriSystem载体特征 载体简介 载体序列 LOCUS pQE-TriSystem 5766 bp DNA circular SYN DEFINITION pQE-TriSystem ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers source 1..5766 /organism="pQE-TriSystem" /mol_type="other DNA" misc_feature 68..355 /label="CAG_enhancer" CDS complement(456..983) /label="ORF frame 2" misc_feature 1012..1031 /label="pCAG_F_primer" terminator 1743..1778 /label="T7_terminator" misc_feature complement(1801..1818) /label="Baculovirs_rev_primer" misc_feature 1808..1885 /label="3_AcPH" CDS complement(1915..3168) /label="ORF frame 1" misc_feature complement(1997..2795) /label="r_ORF1629" gene complement(4015..4875) /label="Ampicillin" /gene="Ampicillin" CDS complement(4015..4875) /label="ORF frame 1" CDS complement(5103..5708) /label="ORF frame 2" ORIGIN 1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT 61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC 121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG 181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT 241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT 361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT 421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG 481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA 541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG 601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC 661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG 721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA 781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG 841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT 901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA 961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC 1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA 1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC 1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC 1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA 1261 TATACCATGG CGATATCCCG GGAGCTCGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG 1321 CAGGTCGACG GTACCGGTTC GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCCAG 1381 CCAGAACTCG CCCCGGAAGA CCCCGAGGAT CTCGAGCACC ACCATCACCA TCACCATCAC 1441 TAAGTGATTA ACCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG 1501 CCCTGGCTCA CAAATACCAC TGAGATCGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC 1561 ATCATGAAGC CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA 1621 ATAGTGTGTT GGAATTTTTT GTGTCTCTCA CTCGGAAGGA CATATGGGAG GGCAAATCAT 1681 TTAAAACATC AGAATGAGTT TTTGGTTTAG AGTTTGGCAA CATATGCCCA TATGTAACTA 1741 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGCATGCGGA 1801 GGAAATTCTC CTTGAAGTTT CCCTGGTGTT CAAAGTAAAG GAGTTTGCAC CAGACGCACC 1861 TCTGTTCACT GGTCCGGCGT ATTAAAACAC GATACATTGT TATTAGTACA TTTATTAAGC 1921 GCTAGATTCT GTGCGTTGTT GATTTACAGA CAATTGTTGT ACGTATTTTA ATAATTCATT 1981 AAATTTATAA TCTTTAGGGT GGTATGTTAG AGCGAAAATC AAATGATTTT CAGCGTCTTT 2041 ATATCTGAAT TTAAATATTA AATCCTCAAT AGATTTGTAA AATAGGTTTC GATTAGTTTC 2101 AAACAAGGGT TGTTTTTCCG AACCGATGGC TGGACTATCT AATGGATTTT CGCTCAACGC 2161 CACAAAACTT GCCAAATCTT GTAGCAGCAA TCTAGCTTTG TCGATATTCG TTTGTGTTTT 2221 GTTTTGTAAT AAAGGTTCGA CGTCGTTCAA AATATTATGC GCTTTTGTAT TTCTTTCATC 2281 ACTGTCGTTA GTGTACAATT GACTCGACGT AAACACGTTA AATAGAGCTT GGACATATTT 2341 AACATCGGGC GTGTTAGCTT TATTAGGCCG ATTATCGTCG TCGTCCCAAC CCTCGTCGTT 2401 AGAAGTTGCT TCCGAAGACG ATTTTGCCAT AGCCACACGA CGCCTATTAA TTGTGTCGGC 2461 TAACACGTCC GCGATCAAAT TTGTAGTTGA GCTTTTTGGA ATTATTTCTG ATTGCGGGCG 2521 TTTTTGGGCG GGTTTCAATC TAACTGTGCC CGATTTTAAT TCAGACAACA CGTTAGAAAG 2581 CGATGGTGCA GGCGGTGGTA ACATTTCAGA CGGCAAATCT ACTAATGGCG GCGGTGGTGG 2641 AGCTGATGAT AAATCTACCA TCGGTGGAGG CGCAGGCGGG GCTGGCGGCG GAGGCGGAGG 2701 CGGAGGTGGT GGCGGTGATG CAGACGGCGG TTTAGGCTCA AATGTCTCTT TAGGCAACAC 2761 AGTCGGCACC TCAACTATTG TACTGGTTTC GGGCGCCGTT TTTGGTTTGA CCGGTCTGAG 2821 ACGAGTGCGA TTTTTTTCGT TTCTAATAGC TTCCAACAAT TGTTGTCTGT CGTCTAAAGG 2881 TGCAGCGGGT TGAGGTTCCG TCGGCATTGG TGGAGCGGGC GGCAATTCAG ACATCGATGG 2941 TGGTGGTGGT GGTGGAGGCG CTGGAATGTT AGGCACGGGA GAAGGTGGTG GCGGCGGTGC 3001 CGCCGGTATA ATTTGTTCTG GTTTAGTTTG TTCGCGCACG ATTGTGGGCA CCGGCGCAGG 3061 CGCCGCTGGC TGCACAACGG AAGGTCGTCT GCTTCGAGGC AGCGCTTGGG GTGGTGGCAA 3121 TTCAATATTA TAATTGGAAT ACAAATCGTA AAAATCTGCT ATAAGCATTG TAATTTCGCT 3181 ATCGTTTACC GTGCCGATAT TTAACAACCG CTCAATGTAA GCAATTGTAT TGTAAAGAGA 3241 TTGTCTCAAG CTCGGAACGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 3301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 3361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 3421 TAGGCTCCGC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 3481 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 3541 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 3601 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 3661 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 3721 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 3781 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 3841 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 3901 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 3961 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGTTACCA 4021 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 4081 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 4141 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 4201 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 4261 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 4321 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 4381 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 4441 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 4501 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 4561 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 4621 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 4681 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 4741 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 4801 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 4861 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 4921 TCTCATGTCC GCGCGTTTCC TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC 4981 AAACTGCCAA AAAATGAAAA CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG 5041 TCTCAATCCT ATTTGTAATT ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT 5101 TATTAACGAT ACAAACCAAA CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC 5161 GCGTAGTTAT AATCGCTGAG GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT 5221 TGCGACAATA TAATTTTATT TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT 5281 TCCTTCTCTT TTTCATTTTT CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT 5341 ATGTATCTAT CGTATAGAGT AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG 5401 GGGTGTATAG TACCGCTGCG CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT 5461 AGTTCTTCGG AGTGTGTTGC TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG 5521 TCGGTTTTGT ACAATATGTT GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA 5581 TTTTTTAGCA GCACCGGATT AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA 5641 AACAGTTCAC CTCCCTTTTC TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA 5701 ACAGCCATTG TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA 5761 TAGTTG ​