pFUSE-hlgG1e1-Fc1 BioVector NTCC质粒载体菌种细胞基因保藏中心

pFUSE-hlgG1e1-Fc1 BioVector NTCC质粒载体菌种细胞基因保藏中心 GENERAL PRODUCT USE pFUSE-Fc is a family of plasmid developed to facilitate the construction of Fc-fusion proteins by fusing the effector region of a protein to the Fc region of an immunoglobulin G (IgG). pFUSE-Fc plasmids yield high levels of Fc-fusion proteins. The level of expression is usually in the µg/mL range. They can be transfected in a variety of mammalian cells, including myeloma cell lines, CHO cells, monkey COS cells and human embryonic kidney (HEK)293 cells, cells that are commonly used in protein purification systems. pFUSE-Fc plasmids allow the secretion of Fc-Fusion proteins. As FcFusion proteins are secreted, they can be easily detected in the supernatant of pFUSE-Fc-transfected cells by SDS-PAGE. Furthermore, functional domains can be identified by immunoblotting and ligand blotting. Fc-Fusion proteins can be easily purified by single-step protein A o r protein G affinity chromatography. InvivoGen provides pFUSE-Fc vectors featuring Fc regions from d i fferent species and isotypes. In humans, there are four isotypes: Ig G 1 , IgG2, IgG3 and IgG4. The Fc region mediates effector functions, such as antibody-dependent cellular cytotoxicity (ADCC) and complementdependent cytotoxicity (CDC). IgG isoforms exert different levels of e ffector functions increasing in the order of IgG4

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