E.coli dsbA突变株 BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli dsbA突变株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Biovector595591 E.coli DsbA mutant E.coli DsbA mutant, 1vial, Storage:4℃

DsbA plays a central role in periplasmic protein folding. A dsbA mutant of an E. coli strain exhibits numerous in vivo phenotypes, including a loss of motility, an absence of alkaline phosphatase activity, sensitivity to benzylpenicillin and dithiothreitol, and resistance to phage M13, because of severe defects in disulfide bond formation in proteins requiring these processes. In addition, dsbA-null mutants have a decreased growth rate in minimal media, as compared with the wild-type strain, and show mucoid colonies when grown on plates in minimal media

Name: dsbA
Type: Gene Synonyms

b3860 MG1655 locus tag
iarA increased ampicillin resistance, Alksne et al.
ppfA


Mnemonic: disulfide bond
Left End Point: 87.11
Right End Point: 87.12Products:

disulfide oxidoreductase, periplasmic
protein disulfide-isomerase


Direction: >
Priority: 03 Alleles of This Gene

dsbA1::kan
dsbA723(del)::kan
dsbA791-YFP(::cat)


External Database Links:Host Site Page Links
EcoGene.org EG11297
EcoliWiki dsbA


Comment:
essential role in cytochrome c synthesis and formate-dependent nitrite reduction. See Metheringham et al. Direct donor of disulfides to newly synth. periplasmic proteins
References:
Bardwell, J.C., K. McGovern, J.R. Beckwith 1991. Identification of a protein required for disulfide bond formation in vivo. Cell 67:581-589
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