Nitrosospira multiformis BioVector NTCC质粒载体菌种细胞基因保藏中心

Nitrosospira multiformis BioVector NTCC质粒载体菌种细胞基因保藏中心 Nitrosospira multiformis
Strain Designations C 71
Isolation Soil, Suriname
Biosafety Level 1
Type Strain yes
Comments Morphology and DNA content
Genome sequenced strain
When propagating this strain, it is imperative that the medium be prepared according to directions in clean glassware. Medium may be dispensed into tissue culture flasks rather than glass flasks to avoid trace contaminants sometimes found on glassware.

Growth Conditions Temperature: 26°C with shaking
Atmosphere: Aerobic
Name of Depositor SW Watson
Isolation Soil, Suriname
References Watson SW, et al. A lobular ammonia-oxidizing bacterium Nitrosolobus multiformis nov. sp.. Arch. Mikrobiol. 76: 83-203, 1971. PubMed: 5548293
Medium: Nitrosolobus medium
Ammonium sulfate.........................1.32 g
MgSO4 . 7H2O ...........................380.0 mg
CaCl2 . 2H2O ............................20.0 mg
MnCl2 . 4H2O ...........................200.0 mcg
Na2MoO4 . 2H2O..........................100.0 mcg
CoCl2 . 6H2O .............................2.0 mcg
ZnSO4 . 7H2O ...........................100.0 mcg
K2HPO4 ..................................87.0 mg
Phenol red 0.5%..........................0.25 ml
Chelated iron (Geigy Chem. 13% iron).....1.0 mg
Distilled water..........................1.0 L
Adjust final pH to 7.5 with 0.5 M K2CO3.
Autoclave at 121C for 15 minutes.
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