pBABE-puro-DN-hTERT BioVector NTCC质粒载体菌种细胞基因保藏中心

pBABE-puro-DN-hTERT BioVector NTCC质粒载体菌种细胞基因保藏中心 Vector backbone pBABE-puro (Search Vector Database) Backbone size w/o insert (bp) 5169 Vector type Mammalian Expression, Retroviral Selectable markers Puromycin GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name hTERT Alt name telomerase reverse transcriptase Species H. sapiens (human) Insert Size (bp) 3500 Mutation Dominant negative, 2 base pair mutation, catalytically dead Entrez Gene TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site EcoRI (not destroyed) 3′ cloning site SalI (not destroyed) 5′ sequencing primer pBABE 5' [Supplier来源] http://www.biovector.net