pRS300植物miRNA表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRS300植物miRNA表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 Backbone for expressing plant artificial miRNAs. Vector backbone pBluescriptSK+ (Search Vector Database) Backbone size w/o insert (bp) 2964 Vector type Plant Expression, RNAi ; Plant gene inactivation GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name MIR319a Species A. thaliana (mustard weed) Insert Size (bp) 404 Entrez Gene MIR319a (a.k.a. AT4G23713, JAGGED AND WAVY, JAW, MICRORNA 319, MIR319, MIR319A, microRNA319A, p_MI0000544) CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site Sma1 (destroyed during cloning) 3′ cloning site Sma1 (destroyed during cloning) 5′ sequencing primer T7 3′ sequencing primer T3. BioVector NTCC Inc. [Supplier来源] http://www.biovector.net