KCL22 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KCL22 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 Cat No.: NTCC592181 Cell line: KCL-22 Species: human (Homo sapiens) Cell type: chronic myeloid leukemia in blast crisis Origin: established from the pleural effusion of a 32-year-old woman with Philadelphia chromosome-positive CML in blast crisis in 1981; described to contain the t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene and a p53 mutation Reference(s): 14690, 14691, 14484 Biosafety level: 1 Permissions and restrictions: A Cell Culture Data: Morphology: round, single cells in suspension, sometimes in small clusters; Medium: 90% RPMI 1640 + 10% h.i. FBS Subculture: split saturated culture 1:5 to 1:10 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.5 x 106 cells/ml; maximal density of ca. 2.0 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 24 hours Harvest: cell harvest of about 1.0-1.5 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO Scientific Data: Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent (MRA), then negative in microbiological culture, RNA hybridization, PCR assays Immunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD25 -, HLA-DR -; Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human by cytogenetics Cytogenetics: human hyperdiploid karyotype with 3.3% polyploidy - 51(47-51)<2n>X, -X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11) - carries Phx2 - matches published karyotype Molec. Genetics: expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - [Supplier来源] http://www.biovector.net