Y2HGold strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Y2HGold strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 NTCC591285 Y2HGold strain Y2HGold strain, 1vial, Storage:4℃ Description The Saccharomyces cerevisiae strain Y2HGold is designed for use with Clontech's Matchmaker. Gold Yeast Two-Hybrid System. Y2HGold includes the sensitive Aureobasidin A antibiotic resistance gene as one of four reporters—providing exceptionally stringent yeast two-hybrid (Y2H) screening. This strain contains distinct ADE2, HIS3, MEL1, and AUR1-C reporter constructs that are only expressed in the presence of GAL4-based protein interactions. Y2HGold is extremely effective in minimizing false positive protein interactions and background during a typical GAL4-based two-hybrid screen. The nutritional requirements (i.e., auxotrophic markers) of Y2HGoldYeast Strain were • verified by streaking samples onto six types of SD minimal media: SD/-Ade, SD/-His,SD/-Leu, SD/-Trp, SD/-Ura, and SD/-Met. Reporter function. The function of the • ADE2, HIS3, MEL1 and AUR1-C reporters in Y2HGold Yeast Strain were verified using positive and negative control plasmids and plating on minimal media lacking adenine and histidine, and containing X-alpha-Gal and Aureobasidin A. [Supplier来源] http://www.biovector.net