RK-13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

RK-13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 RK13 Cells Cat No.: NTCC513992 Cell Line Name: RK13 Keywords: Rabbit kidney Cell Line Description: RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line. Species: Rabbit Tissue of Origin: Kidney CellType: Epithelial-like Growth Mode: Adherent Karyotype: 2n=44, stem-line = 56 (=22%) Biosafety Information: 2 Subculture Routine: Split sub-confluent cultures (70-80%) seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical. Culture Medium: MEM(HBSS)+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Depositor: Dr. R. Riebe, Collection of Cell Lines in Veterinary Medicine (CCLV) Federal Research Centre for Virus Diseases of Animals, 17498 Island Riems, Germany Originator: No Country: Germany References: Lancet 1963; 2:640 [Supplier来源] http://www.biovector.net