青海弧菌Q67 Vibrio qinghaiensis sp. Q67 BioVector NTCC质粒载体菌种细胞基因保藏中心

青海弧菌Q67 Vibrio qinghaiensis sp. Q67  

BioVector NTCC质粒载体菌种细胞基因保藏中心                      

NTCC595402 Vibrio qinghaiensis sp. Q67  

Storage:4℃  

Description描述

Of the methods for ecotoxicity assessment, bioassays using luminescent bacteriaare widely applied in water toxicity tests for its advantages of goodsensibility, reproducibility, flexibility and low cost (Parvez et al., 2006;Mendonça et al., 2009). The luminescent bacteria assays often show goodcorrelations to toxicity bioassays using other flora and fauna such as algae,crustacean and fish (Girotti et al., 2008). As an alternative luminescentbacterium, Vibrio qinghaiensis sp. Q67(abbreviated as “Q67”) has been isolated from the body surface of Cymnocyprisprzewalskii, one of the edible fish in Qinghai Province, China (Zhu et al.,1994). It is verified that Q67 has similar property to other luminescentbacteria in light emission and inhibition, and can be used for the bioassayunder a freshwater environment which much benefits the toxicity tests in mostcases (Ma et al., 1998). Nowadays, both bioassays using Photobacteriumphosphoreum T3 spp. (marine strain) and Q67 are recommended as applicablemethods for ecotoxicity tests.

      Culture medium:

KH2PO427. 2mg ,

Na2HPO4· 12H2O 71. 6mg,

MgSO4 ·7H2O  0. 5g,

MgCl2 ·6H2O 1. 22g,

CaCl266.0 mg,

NaHCO3   2. 68g,

NaCl  3. 08g,

yeastextract paste 5. 0g,

tryptone5. 0g,

glycerol3. 0g,

distilledwater 1000mL

adjust pH using 1mol / L NaOH to 8.5 ± 0.5

autoclavesterilization under 121 ° C, 20min

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