E.coli HB2151 strain噬菌体展示系统可溶表达宿主菌 BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli HB2151 strain噬菌体展示系统可溶表达宿主菌 BioVector NTCC质粒载体菌种细胞基因保藏中心 NTCC-HB2151 HB2151 HB2151, 500uL. Resistance:nalidixic acid. Storage:4℃ Description HB2151 is a non-amber suppressor E. coli strain used for expressing foreign proteins without the pIII fusion moiety. Prepare a stock culture in 20% glycerol after receipt and maintain a working stock plate of HB2151 on M9/+Thi minimal medium to keep selection on the F' episome. HB2151 genotype nalr thi-1 ara (lac-proAB) [F' proAB+lacIq lacZM15] Recovery 1. Obtain an LB agar plate with the appropriate antibiotic. 2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.) 3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1. 4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2. 5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3. 6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet). 7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net