Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC) BioVector NTCC质粒载体菌种细胞基因保藏中心

Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC) BioVector NTCC质粒载体菌种细胞基因保藏中心 Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Vascular Cell Basal Media supplemented with Endothelial Cell Growth Kit components, provide an ideal cell system to propagate HUVEC in low serum conditions with or without the addition of human recombinant VEGF. The cells are cryopreserved in the first passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are qualitytested together to guarantee optimum performance and reliability. Components: One vial Primary Umbilical Vein Endothelial Cells, Normal, Human (ATCC® PCS100010 ™) containing a minimum of 5 x 105 viable cells (provided). Also Required: A. One bottle of Vascular Cell Basal Medium (ATCC PCS100030) plus one Endothelial Cell Growth Kit of either: 1. Endothelial Cell Growth KitBBE (ATCC PCS100040) containing each of the following growth supplements: Bovine Brain Extract, rh EGF, Lglutamine, heparin sulfate, hydrocortisone hemisuccinate, Fetal Bovine Serum, and ascorbic acid. 2. Endothelial Cell Growth KitVEGF (ATCC PCS100041) containing each of the following growth supplements: rh VEGF, rh EGF, rh FGF basic, rh IGF1, ascorbic acid, Lglutamine, heparin sulfate, hydrocortisone hemisuccinate and Fetal Bovine Serum. B. Optional media supplements 1. GentamicinAmphotericin B Solution (ATCC PCS999025) 2. PenicillinStreptomycinAmphotericin B Solution (ATCC PCS999002) 3. Phenol Red (ATCC PCS999001) C. Reagents for subculture 1. DPBS (ATCC 302200) 2. TrypsinEDTA for Primary Cells (ATCC PCS999003) containing 0.05% Trypsin and 0.02% EDTA. Note:Do not use other TrypsinEDTA concentrations with ATCC PCS100010 ™. 3. Trypsin Neutralizing Solution (ATCC PCS999004) Cell Characteristics Tissue: Umbilical Morphology: Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells. Growth Properties: Adherent [Supplier来源] http://www.biovector.net