pwtCas9-bacteria BioVector-044250 BioVector NTCC质粒载体菌种细胞基因保藏中心

pwtCas9-bacteria BioVector-044250 BioVector NTCC质粒载体菌种细胞基因保藏中心 aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown. Vector backbone pUC19 (Search Vector Database) Total vector size (bp) 6932 Vector type Bacterial Expression, CRISPR GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) Top10 Copy number High Copy GENE/INSERT Gene/Insert name wild-type Cas9 Species S .pyogenes Insert Size (bp) 4107 Promoter pLtetO-1 CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site BglII (not destroyed) 3′ cloning site XhoI (not destroyed) 5′ sequencing primer agaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGATAAGAAATACTCAATAGG 3′ sequencing primer ggagatccttactcgagttaGTCACCTCCTAGCTGACTCAAATCAAT. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net