BRL 3A cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BRL 3A cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 BRL 3A cell line Cat No.: NTCC595331 Cell Line Name: BRL 3A Keywords: Buffalo Rat liver Cell Line Description: Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA). Species: Rat Tissue of Origin: liver CellType: Epithelial Growth Mode: Adherent Karyotype: Not specified Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: DMEM-H+10%FBS Country: USA References: Proc Natl Acad Sci, USA 1969;62:852; J Cell Physiol 1973;81:153; Cell 1977;11:441 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net