pAG414GPD-dCas9-VPR BioVector-063801 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAG414GPD-dCas9-VPR BioVector-063801 BioVector NTCC质粒载体菌种细胞基因保藏中心 pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector. Cas9 contains D839A and N863A mutations relative to reference sequence (WP_010922251.1). Depositor states that these mutations do not affect function. Vector backbone pAG414GPD (Search Vector Database) Vector type Yeast Expression, CRISPR, Synthetic Biology Selectable markers TRP1 GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH10B Copy number High Copy GENE/INSERT Gene/Insert name dCas9-VPR Alt name VP64-p65-Rta Species S. cerevisiae (budding yeast) Insert Size (bp) 5733 Promoter GPD Tag / Fusion Protein VPR (VP64-p65-Rta) (C terminal on insert) CLONING INFORMATION Cloning method Gateway Cloning 5′ sequencing primer cggtaggtattgattgtaattctg. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net