pAWG-dCas9-VPR BioVector-063802 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAWG-dCas9-VPR BioVector-063802 BioVector NTCC质粒载体菌种细胞基因保藏中心 SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector. Please note that this plasmid contains a stop codon between dCas9-VPR and eGFP and does NOT produce a GFP fusion protein. Cas9 contains D839A and N863A mutations. Depositor states that these mutations should not affect function..Vector backbone pAWG (Search Vector Database) Vector type Insect Expression, CRISPR, Synthetic Biology GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number Unknown GENE/INSERT Gene/Insert name SP-dCas9-VPR Alt name VP64-p65-Rta Species D. melanogaster (fly) Insert Size (bp) 5733 Promoter act5c Tag / Fusion Protein VPR (VP64-p65-Rta) (C terminal on insert) CLONING INFORMATION Cloning method Gateway Cloning 5′ sequencing primer ATCGGCGAACAATTCATACC [Supplier来源] http://www.biovector.net