Mycobacterium smegmatis bacteriophage BioVector NTCC质粒载体菌种细胞基因保藏中心

Mycobacterium smegmatis bacteriophage BioVector NTCC质粒载体菌种细胞基因保藏中心 Mycobacterium smegmatis bacteriophage Host Organism Mycobacterium smegmatis strain Milch Biosafety Level 1 Medium Middlebrook 7H10 with OADC Middlebrook 7H10………………………………………….….19.0 g Glycerol…………………………………………………….…...5.0 ml Dubos Oleic Albumin Complex (OADC) (see below)……...100.0 ml DI Water………………………………………………….……..900.0 ml Add and mix all components except for OADC. Bring to a boil to dissolve agar and autoclave at 121ºC. Cool to 50-55ºC and aseptically OADC. OADC Oleic Acid (Sigma Cat # O-1008)……………………………..0.05 g Albumin Fraction V Bovine (Sigma A9647)……….………...5 g Dextrose (BD 215530)…………………………………………2 g Catalase (beef) (Sigma C-9322)……………………………...0.004 g NaCl……………………………………………………………..0.85 g DI Water…………………………………………………………100 ml OADC is available from BD (237510) or can be made following the formulation above. Growth Conditions Temperature: 37°C Atmosphere: Aerobic Name of Depositor G Penso BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net