pDL12AI枯草芽孢杆菌整合型温敏分泌表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pDL12AI枯草芽孢杆菌整合型温敏分泌表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 pDL12AI vector contains the cold-inducible des promoter of Bacillus subtilis. It allows for secretion of recombinant proteins without the need for a cost-intensive inductor. When mid-exponential phase bacterial cells are rapidly transferred from 37 °C to 25 °C or even a lower temperature, the synthesis of most cellular proteins greatly decreases, while that of cold-shock proteins is transiently upregulated (Weber and Marahiel, 2003). In Bacillus subtilis, one of these cold-shock proteins is a membrane-bound desaturase (Δ5-Des) encoded by the des gene (Aguilar et al., 1998). This enzyme catalyzes the introduction of a cis double bond at the Δ5 position of a wide variety of saturated fatty acids. It has been shown that the des gene is tightly regulated during cold shock. Production of recombinant proteins started within the first 30 min after temperature downshock to 25 °C and continued for about 5 h (Thuy Le and Schumann 2007). [Supplier来源] http://www.biovector.net