TZM-bl cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

TZM-bl cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心        

TZM-BL细胞是一种携带有荧光素酶报告基因的传代细胞系，并且该报告基因受HIV-1 tat原件调控，只有在HIV-1 Tat蛋白存在的情况下才能有效表达。利用HIV-1病毒感染该细胞后，表达Tat蛋白，从而上调荧光素酶基因表达，继而通过检测荧光素酶的表达情况以判断HIV-1的感染与复制效率。若药物具有抑制HIV-1的作用，则荧光素酶的表达将显著下降。    

Special Characteristics: The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either β-gal or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran in the infection medium. The ß-gal and luciferase genes are also induced by HIV-2 infection.
Cell Type: TZM-bl, previously designated JC53-bl (clone 13) is a HeLa cell line. The parental cell line (JC.53) stably expresses large amounts of CD4 and CCR5. The TZM-bl cell line was generated from JC.53 cells by introducing separate integrated copies of the luciferase and ß-galactosidase genes under control of the HIV-1 promoter. The TZM-bl cell line is highly sensitive to infection with diverse isolates of HIV-1.
Freeze Medium: 50% FBS, 40% DMEM, 10% DMSO.
Growth Characteristics: Adherent cell line. No special requirements for thawing and reestablishing the culture. Cells grow in single cell layer. Antibiotic selection is not required to maintain stable expression of the receptors and reporter genes.
Propagation Medium: DMEM (90%), 10% FBS, 100 units of Penicillin and 0.1 mg/ml of Streptomycin.
Sterility: Negative for bacteria, mycoplasma, and fungi.
References: Platt EJ, Bilska M, Kozak SL, Kabat D, Montefiori DC. Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1. J Virol.2009;83(16):8289-92.

Takeuchi Y, McClure MO, Pizzato M. Identification of γ-retroviruses constitutively released from cell lines used for HIV research. J Virol 82(24):12585-8, 2008 Abstract

Wei X, Decker JM, Liu H, Zhang Z, Arani RB, Kilby JM, Saag MS, Wu X, Shaw GM, Kappes JC. Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy. Antimicrob Agents Chemother 46:1896-1905, 2002.

Derdeyn CA, Decker JM, Sfakianos JN, Wu X, O'Brien WA, Ratner L, Kappes JC, Shaw GM, Hunter E. Sensitivity of human immunodeficiency virus type 1 to the fusion inhibitor T-20 is modulated by coreceptor specificity defined by the V3 loop of gp120. J Virol74:8358-8367, 2000.

Platt EJ, Wehrly K, Kuhmann SE, Chesebro B, Kabat D. Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1. J Virol 72:2855-2864, 1998.

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