pBAD-ompA-RGD BioVector NTCC质粒载体菌种细胞基因保藏中心

pBAD-ompA-RGD BioVector NTCC质粒载体菌种细胞基因保藏中心 L-Arabinose-induced OmpARGD expression by surface-engineered S. typhimurium (ΔppGppRGD). (A) A topological model of S. typhimurium OmpA after insertion of the RGD sequence (ACDCRGDCFCG), and its secondary structure. The image is based on the expected RGD sequence/OmpA structure (Protein Data Bank (PDB) ID code 2GE4). (B) A map of the bacterial expression plasmid (pOmpARGD). (C) The ppGpp-defective S. typhimurium strain (ΔppGpp) was transformed with pOmpARGD (ΔppGppRGD). Protein production by ΔppGpp, ΔppGpp ΔompA, and ΔppGppRGD strains was induced by L-arabinose. The expression of OmpA (37 kDa) was analyzed by Western blotting with an anti-major outer membrane protein (MOMP ) antibody. The position of OmpARGD is indicated by an arrow. (D) The growth of S. typhimurium was assessed using the wild type or in untransformed ΔppGpp or transformed ΔppGppRGD after induction of OmpARGD expression. To induce the pBAD system in cultured bacteria, 0.2% L-arabinose was added to cultures at mid-log phase (1 h after starting the culture). The A600 was determined every 1 or 1.5 h for a period of 10 h. Results are representative of at least three independent experiments. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net