HIV-H2BmRFP， pHIV-H2BmRFP慢病毒表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

HIV-H2BmRFP， pHIV-H2BmRFP慢病毒表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心 The HIV-H2BmRFP plasmid was cloned by replacing Zsgreen in HIV-Zsgreen with H2BmRFP to generate a bicistronic lentiviral vector. cDNA can be cloned into the multiple cloning site (HpaI, XbaI, SmaI) in order to coexpress the inserted gene with H2BmRFP. • Vector backbone HIV-Zsgreen • Backbone size w/o insert (bp)8046 • Vector type Mammalian Expression, Lentiviral GROWTH IN BACTERIA • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) Stbl3 • Growth instructions Growth in Stbl3 bacteria is preferred. • Copy number High Copy GENE/INSERT • Gene/Insert name Histone H2B monomeric red fluorescent protein • Alt name H2BmRFP • Alt name H2B-mRFP • Species Discosoma coral • Insert Size (bp) 1065 CLONING INFORMATION • Cloning method Restriction Enzyme • 5′ sequencing primer5'-TGGAATTTGCCCTTTTTGAG-3' • 3′ sequencing primer5'-AGGAACTGCTTCCTTCACGA-3' BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net