pGL3-basic报告载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGL3-basic报告载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation. The pGL3-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc+. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luc+ gene. pGL3-Basic Vector circle map. Additional description: luc+, cDNA encoding the modified firefly luciferase; Ampr, gene conferring ampicillin resistance in E. coli; f1 ori, origin of replication derived from filamentous phage; ori, origin of replication in E. coli. Arrows within luc+ and the Ampr gene indicate the direction of transcription; the arrow in the f1 ori indicates the direction of ssDNA strand synthesis. pGL3-Basic Vector Sequence Reference Points: Promoter (none) Enhancer (none) Multiple cloning region 1–58 Luciferase gene (luc+) 88–1740 GLprimer2 binding site 89–111 SV40 late poly(A) signal 1772–1993 RVprimer4 binding site 2080–2061 ColE1-derived plasmid replication origin 2318 β-lactamase gene (Ampr) 3080–3940 f1 origin 4072–4527 upstream poly(A) signal 4658–4811 RVprimer3 binding site 4760–4779 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net