pDL-Pdes-SamyQ-intein BioVector NTCC质粒载体菌种细胞基因保藏中心

pDL-Pdes-SamyQ-intein BioVector NTCC质粒载体菌种细胞基因保藏中心 pDL12AI，pDL-Pdes-SamyQ-intein枯草芽孢杆菌整合型温度诱导分泌表达自切割载体质粒，基因组整合型过表达，26度低温诱导表达型启动子，分泌型表达标签，标签表达后自切割，非常适合枯草芽孢杆菌整合型表达。Amp大肠杆菌抗性，Kan枯草芽孢杆菌抗性。amyE整合位点。BioVector NTCC保藏中心开发提供。 pDL12AI vector contains the cold-inducible des promoter of Bacillus subtilis. It allows for secretion of recombinant proteins without the need for a cost-intensive inductor. When mid-exponential phase bacterial cells are rapidly transferred from 37 °C to 25 °C or even a lower temperature, the synthesis of most cellular proteins greatly decreases, while that of cold-shock proteins is transiently upregulated (Weber and Marahiel, 2003). In Bacillus subtilis, one of these cold-shock proteins is a membrane-bound desaturase (Δ5-Des) encoded by the des gene (Aguilar et al., 1998). This enzyme catalyzes the introduction of a cis double bond at the Δ5 position of a wide variety of saturated fatty acids. It has been shown that the des gene is tightly regulated during cold shock. Production of recombinant proteins started within the first 30 min after temperature downshock to 25 °C and continued for about 5 h (Thuy Le and Schumann 2007). [Supplier来源] http://www.biovector.net