BPK3079 BioVector-078741 BioVector NTCC质粒载体菌种细胞基因保藏中心

BPK3079 BioVector-078741 BioVector NTCC质粒载体菌种细胞基因保藏中心 Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites) .Use BsmBI sites to insert crRNA spacer sequence into the vector. This plasmid will express gRNA in mammalian cells for Cpf1 from Acidaminococcus sp. (strain BV3L6). Recommended to use with SQT1659 (plasmid# 78743), which expresses Acidaminococcus sp. Cpf1. Vector backbone MLM3636 (Search Vector Database) Backbone manufacturer Joung lab (Addgene BioVector-0 43860) Vector type Mammalian Expression, CRISPR GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name AsCpf1 crRNA backbone, without spacer sequence Species Synthetic Promoter U6 CLONING INFORMATION Cloning method Restriction Enzyme 5′ cloning site BsmBI (not destroyed) 3′ cloning site BsmBI (not destroyed) 5′ sequencing primer OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3') BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net