pTE4396 BioVector-074041 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTE4396 BioVector-074041 BioVector NTCC质粒载体菌种细胞基因保藏中心 Expresses human codon-optimized AsCpf1 and As crRNA. Vector backbone pcDNA3.1 (Search Vector Database) Backbone size w/o insert (bp) 4642 Total vector size (bp) 9677 Vector type Mammalian Expression, CRISPR Selectable markers Neomycin (select with G418) GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) NEB Stable Copy number High Copy GENE/INSERT 1 Gene/Insert name As crRNA Alt name Acidaminococcus sp. CRISPR RNA Insert Size (bp) 47 Promoter human U6 CLONING INFORMATION FOR GENE/INSERT 1 Cloning method Unknown 5′ sequencing primer AAAACAGGAAGGCAAAATGC (Common Sequencing Primers) GENE/INSERT 2 Gene/Insert name AsCpf1 Alt name Acidaminococcus sp. Cpf1 nuclease Insert Size (bp) 3921 Promoter CMV Tags / Fusion Proteins NLS (C terminal on insert) 3xHA (C terminal on insert) CLONING INFORMATION FOR GENE/INSERT 2 Cloning method Unknown 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net