SQT1665 BioVector-078744 BioVector NTCC质粒载体菌种细胞基因保藏中心

SQT1665 BioVector-078744 BioVector NTCC质粒载体菌种细胞基因保藏中心 Human expression plasmid for LbCpf1-NLS-3xHA. This plasmid expresses Cpf1 from Lachnospiraceae bacterium ND2006 in mammalian cells. Recommended to use with BPK3082 (plasmid# 78742), which can be used to express a gRNA for LbCpf1. Vector backbone pCAG-GFP (Search Vector Database) Backbone manufacturer Cepko lab (Addgene plasmid# 11179) Vector type Mammalian Expression, CRISPR GROWTH IN BACTERIA Bacterial Resistance(s) Ampicillin Growth Temperature 37°C Growth Strain(s) DH5alpha Copy number High Copy GENE/INSERT Gene/Insert name mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA Alt name Cpf1 Species Lachnospiraceae bacterium ND2006 Promoter CAG Tags / Fusion Proteins NLS (C terminal on insert) 3x HA (C terminal on insert) CLONING INFORMATION Cloning method Unknown 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3') 3′ sequencing primer M13-rev BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net