Enterococcus faecalis V583 粪肠球菌V583 BioVector NTCC质粒载体菌种细胞基因保藏中心

Enterococcus faecalis V583 粪肠球菌V583

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Enterococcus faecalis V583Strain Designations V583Isolation Human Blood from a patient, St. Louis Missouri, United States, 1987Biosafety Level 2Genome Sequenced Strain Enterococcus faecalis V583, complete genomeType Strain noAntibiotic Resistance Resistant to gentamicins gentamicinResistant to vancomycinSensitive to daptomycinSensitive to streptomycinComments Genome sequenced strainPresence of vanB gene confirmed by PCR.Sensitive to streptomycinResistant to gentamicinResistant to vancomycinSensitive to daptomycinMedium Medium 44: Brain Heart Infusion Agar/BrothBrain Heart Infusion Agar/Broth Brain Heart Infusion Agar (BD 211065)…………………..52 g Agar Medium DI Water……………………...………………………………1000 ml Autoclave at 121ºC. Brain Heart Infusion Broth (BD 237500)…………..…….37.0g Broth Medium DI Water…………………………………………………….1000 ml Autoclave at 121ºC. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Calf Brains, infusion from…………………………………..200 g Brain Heart Infusion Composition Beef Hearts, infusion from………………………………….250 g Proteose Peptone…………………………………...………10.0 g Dextrose……………………………………………..………..2.0g NaCl…………………………………………………...………5.0g Na2HPO4…………………………………………...………...2.5g DIWater……………………………………………………….1000ml Final pH 7.4 +/- 0.2 *Omit agar for broth medium. Final Product Description  Medium should be tan in color.Recommended storage temperature: 2-8ºC.Growth Conditions Temperature: 37°CAtmosphere: AerobicIsolation Human Blood from a patient, St. Louis Missouri, United States, 1987References Paulsen IT, et al. Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 299(5615): 2071-2074, 2003. PubMed: 12663927Cross References Nucleotide (GenBank) : AE016830 Enterococcus faecalis V583, complete genomeNucleotide (GenBank) : AE016831 Enterococcus faecalis V583 plasmid pTEF2, complete sequenceNucleotide (GenBank) : AE016832 Enterococcus faecalis V583 plasmid pTEF3, complete sequenceNucleotide (GenBank) : AE016833 Enterococcus faecalis V583 plasmid pTEF1, complete sequence        BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net      [Supplier来源]  http://www.biovector.net