pGL3-Enhancer载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pGL3-Enhancer载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation. The pGL3-Enhancer Vector contains an SV40 enhancer located downstream of luc+ and the poly(A) signal. This aids in the verification of functional promoter elements because the presence of an enhancer will often result in transcription of luc+ at higher levels. Figure 1. The pGL3-Enhancer Vector circle map. Additional description: luc+, cDNA encoding the modified firefly luciferase; Ampr , gene conferring ampicillin resistance in E. coli; f1 ori, origin of replication derived from filamentous phage; ori, origin of plasmid replication in E. coli. Arrows within luc+ and the Ampr gene indicate the direction of transcription; the arrow in f1 ori indicates the direction of ssDNA strand synthesis. pGL3-Enhancer Vector Sequence Reference Points: Promoter (none) Multiple cloning region 1–58 Luciferase gene (luc+) 88–1740 GLprimer2 binding site 89–111 SV40 late poly(A) signal 1772–1993 Enhancer 2013–2249 RVprimer4 binding site 2307–2326 ColE1-derived plasmid replication origin 2564 β-lactamase gene (Ampr) 3329–4186 f1 origin 4318–4773 upstream poly(A) signal 4904–5057 RVprimer3 binding site 5006–5025 BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net