pRGE31, pRGEB31, pRGE32 and pRGEB32 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pRGE31, pRGEB31, pRGE32 and pRGEB32 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 (Empty Backbone) Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation. The full plasmid sequence was assembled, the XmaI site is not present. Please use the reference links above to access a published protocol for using plasmids pRGE31, pRGEB31, pRGE32 and pRGEB32 as well as a CRISPR design software tool. Vector backbone pCAMBIA1300 (Search Vector Database) Backbone manufacturer CAMBIA Backbone size (bp) 8958 Modifications to backbone 1. Removed Bsa I sites. 2. Inserted the U3-gRNA-35S-Cas9 cassette from pRGE31. Vector type CRISPR ; plant expression Promoter Rice snoRNA U3 and dual 35S promoter Selectable markers Hygromycin GROWTH IN BACTERIA Bacterial Resistance(s) Kanamycin Growth Temperature 37°C Growth Strain(s) DH5alpha Growth instructions For agrobacterium stranis like EHA105, growth temperature is 28 C. Copy number High Copy CLONING INFORMATION Cloning method Restriction Enzyme 5′ sequencing primer M13R(-48) 3′ sequencing primer M13F(-20) BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net