Christensenella minuta BioVector NTCC中国质粒载体菌种细胞基因保藏中心

Christensenella minuta BioVector NTCC中国质粒载体菌种细胞基因保藏中心 Name: Christensenella minuta Morotomi et al. 2012 NTCC No.: 522607, Type strain Isolated from: human faeces from a healthy Japanese male Geographic origin: Japan, Tokyo Genbank accession numbers: 16S rRNA gene: AB490809 Cultivation conditions: RCM MEDIUM (N2/CO2) Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use, add 2.0 g of sodium bicarbonate per liter medium from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Check pH of medium after autoclaving and adjust pH to 6.8, if necessary. Adjust pH of medium after autoclaving to pH 7.3 – 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere., anaerobic, 37°C Summary and additional information: <- Y. Watanabe, Yakult Central Inst. Microbiol. Res., Tokyo, Japan; YIT 12065 <- F. Nagai and M. Morotomi. Human faeces from a healthy Japanese male; Japan, Tokyo. Type strain.Taxonomy/description (16595). Sequence accession no. 16S rRNA gene: AB490809. (Medium 634c with strain-specific modifications, 37°C, anaerobic). BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net