A7r5大鼠胚胎主动脉平滑肌细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

A7r5大鼠胚胎主动脉平滑肌细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 Organism: Rattus norvegicus, rat Strain: DBIX Tissue: aorta, thoracic/smooth muscle Age: embryo Morphology: fibroblast Growth Properties: adherent Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of TrypsinEDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Comments: The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase. Muscle type CPK is synthesized after cell division has ceased. [Supplier来源] http://www.biovector.net