pBacPAK8 AcMNPV昆虫杆状病毒表达系统载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pBacPAK8 AcMNPV昆虫杆状病毒表达系统载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 Description: Available as part of the BacPAK Baculovirus Expression System (Cat. No. 631402). pBacPAK8 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites that facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK8 has a pUC origin of replication, an M13 origin for single-stranded DNA production, and an ampicillin resistance gene for selection in E. coli. Restriction Map and Multiple Cloning Site (MCS) of pBacPAK8. All sites shown are unique. The Pac l site at the end of the multiple cloning sites provides translational stop codons in all three reading frames. The M13 origin of replication in pBacPAK8 can be used to package the coding strand of the target gene (top strand in the figure) for sequencing and mutagenesis of the insert. pBacPAK8 also has a pUC origin of replication and an ampicillin resistance gene for propagation in E. coli. [Supplier来源] http://www.biovector.net