pLVX-TetOne-Puro-Luc control BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pLVX-TetOne-Puro-Luc control BioVector NTCC中国质粒载体菌种细胞基因保藏中心 When used as part of the Lenti-X™ Tet-One™ Inducible Expression System (Puro), the pLVX-TetOne-Puro Vector Set allows for lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. pLVX-TetOne-Puro is an all-in-one vector that constitutively expresses the Tet-On® 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. There is also a puromycin antibiotic selection marker on this vector. Target cells transduced with LVX-TetOne lentivirus containing your transgene will express high levels of your gene, but only when cultured in the presence of doxycycline. Storage Conditions  Store at –20°C.  Spin briefly to recover contents.  Avoid repeated freeze/thaw cycles. Shelf Life  1 year from date of receipt under proper storage conditions. Storage Buffer  10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0) Concentration  500 ng/μl Propagation in E. coli  Recommended host strain: Stellar™ Competent Cells (Cat. No. 636763).  Selectable marker: Plasmids confer resistance to ampicillin (100 μg/ml) in E. coli hosts.  E. coli replication origin: pUC Quality Control Data Plasmid Identity & Purity  Digestion with the indicated restriction enzymes produced fragments of the indicated sizes on a 0.8% agarose/EtBr gel: Vector Enzyme(s) Size (kb) pLVX-TetOne-Puro Vector MluI PstI & XbaI 9.2 kb 6.9 & 2.3 kb pLVX-TetOne-Puro-Luc Control Vector MluI BamHI & EcoRI 10.9 kb 9.2 & 1.7 kb  Vector identity was confirmed by sequencing.  A260/A280: 1.8–2.0 [Supplier来源] http://www.biovector.net