HL-60 cell line细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

HL-60 cell line细胞株 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 Organism Homo sapiens, human Tissue peripheral blood Cell Type promyeloblast Product Format frozen Morphology myeloblastic Culture Properties suspension Biosafety Level 1 Disease acute promyelocytic leukemia Age 36 years Gender female Ethnicity Caucasian Applications This cell line is a suitable transfection host. Karyotype The stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DM's, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected. Images Derivation HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia. Clinical Data 36 years Caucasian female Receptor Expression complement, expressed Ref Fc, expressed Ref Oncogene myc + Genes Expressed tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid,myc + Cellular Products tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid Tumorigenic Yes Effects Yes, in nude mice (subcutaneous myeloid tumors) Yes, in semi-solid media Comments HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc oncogene expression. Complete Growth Medium The base medium for this cell line is NTCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Do not allow cell concentration to exceed 1 X 106cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Interval: Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C STR Profile D5S818: 12 D13S317: 8,11 D7S820: 11,12 D16S539: 11 vWA: 16 THO1: 7,8 Amelogenin: X TPOX: 8,11 CSF1PO: 13,14 Isoenzymes AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 References Gallagher R, et al. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood 54: 713-733, 1979. PubMed:288488 Collins SJ, et al. Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. Proc. Natl. Acad. Sci. USA 75: 2458-2462, 1978. PubMed: 276884 Collins SJ, et al. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270: 347-349, 1977. 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