pWPXLd慢病毒绿色荧光表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
- 价 格:¥7920
- 货 号:pWPXLd慢病毒绿色荧光表达载体
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pWPXLd慢病毒绿色荧光表达载体
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net
EF1-alpha启动子,Amp抗性。
pWPXLd is essentially pWPXL without the LoxP site in the 3' LTR.
pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter
Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI.
Please note that ClaI in these vectors is blocked by Dam methylation.
The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning.
Map图谱
- Vector backbonepWPXLd(Search Vector Database)
Backbone size (bp)10455
- Vector typeMammalian Expression, Lentiviral
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
- Copy numberHigh Copy
GENE/INSERT
- Gene/Insert nameNone
Tag / Fusion Protein
EGFP
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteMCS - see map (not destroyed)
3′ cloning siteMCS - see map (not destroyed)
5′ sequencing primerSee map
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net
您正在向 biovector.net 发送关于产品 pWPXLd慢病毒绿色荧光表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 的询问
- 公告/新闻