Enterobacteriaphage MS2噬菌体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Enterobacteriaphage MS2噬菌体

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Species:

Enterobacteriaphage MS2

Taxonomy:

Order:Unassigned
Family:Leviviridae
Type species of the genus:Levivirus

Isolate:

MS2

NTCC No.:

PG13767

Straindesignation:

PC-V3463

History:

<- Phabagen

Genbankaccession numbers:

complete genome:NC_001417

Genomics:

Linear ssRNA.Phage has a very small genome (3.569 nucleotides); small stem-loops from theMS2 genome are very important for detection of RNA in living cells ("MS2tagging": interaction of MS2 coat protein with stem-loop structure fromthe phage genome; used for biochemical purification of RNA-protein complexes)

Morphology:

Nonenveloped,isometric, icosahedral, small, tail-less phage, size approx. 25 nm

Life cycle:

virulent phage,lytic cycle

Plaquesdescription:

large turbidplaques

Image:

Application:

Used in Expt. 49in "Sourcebook of Experiments for the Teaching of Microbiology", eds.S.B. Primrose and A.C. Wardlaw, Academic Press (1982)

Risk group:

1

Phagepropagation

Host bacterium:

Escherichiacoli(Migula 1895)Castellani and Chalmers 1919

Cultivationconditions:

NZCYM-MEDIUMplus 2.0 mg of streptomycin per l, 37°C

NZCYM-MEDIUM

Caseinhydrolysate 10.0 g

NaCl 5.0 g

Casaminoacids (Difco) 1.0 g

Yeastextract 5.0 g

MgSO4 x 7H2O 2.0 g

Maltose 2.0g

Distilledwater 1000.0 ml