R1000发根农杆菌菌株Rhizobium sp.-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

R1000发根农杆菌菌株

Rhizobium sp. R1000

CatNo.: NTCC543056

Designation:R1000

Deposited Name: Agrobacterium rhizogenes (Rikeret al.) Conn

Product Description: Formerly known as Rhizobium rhizogenes.

Medium

Medium3: Nutrient agar or nutrient broth

GrowthConditions

Temperature:26°C

Atmosphere:Aerobic

PropagationProcedure

1.Open vial according to enclosed instructions.

2.From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0mL with a Pasteur or 1.0 mL pipette and use to rehydrate the entire pellet.

3.Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.

4. Useseveral drops of this suspension to inoculate an additional broth tube, an agarslant and/or plate.

5.Incubate the tubes and plate at 26°C for 24 to 48 hours.

Comments:

Colonies on #3 agar are cream, circular, entire,mucoid, convex, and translucent.