出品公司: | BioVector NTCC Inc. |
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载体名称: | pCSDest |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | Gateway;限制性内切酶,多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 5808 bp |
5' 测序引物及序列: | SP6 fwd、T3 fwd |
3' 测序引物及序列: | SV40 polyA rev |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCSDest载体能够在多种细胞中进行高水平的瞬表达。pCSDest载体同时也能进行体外转录和翻译。 |
产品目录号: | #22423 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCSDest多系统广宿主表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
- 价 格:¥9852
- 货 号:pCSDest多系统广宿主表达载体
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCSDest 5808 bp DNA SYN DEFINITION pCSDest ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5808 /organism="pCSDest" /mol_type="other DNA" misc_feature 35..52 /label="Sp6_primer" misc_feature 108..227 /label="attR1" misc_feature complement(132..227) /label="attR2" promoter 257..280 /label="lac_promoter" gene 341..1000 /label="CAT/CamR" /gene="CAT/CamR" CDS 341..1000 /label="ORF frame 2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature 1342..1647 /label="ccdB" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature 1688..1788 /label="attR2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1688..1795) /label="attR1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator complement(1867..1986) /label="SV40_PA_terminator" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(1881..1900) /label="EBV_rev_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" promoter complement(2103..2122) /label="T3_promoter" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" promoter complement(2139..2157) /label="M13_reverse_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(2156..2178) /label="M13_pUC_rev_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" promoter complement(2192..2221) /label="lac_promoter" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(3304..4164) /label="Ampicillin" /gene="Ampicillin" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" CDS complement(3304..4164) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(4206..4234) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 5726..5767 /label="CMV_fwd_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG 61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCA AGGATCAACA AGTTTGTACA 121 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC 181 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCATAT TGGCGGCCGC 241 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG 301 TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG 361 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC 421 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC 481 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT 541 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG 601 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG 661 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA 721 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC 781 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT 841 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT 901 GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA 961 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA AGATCTGGAT CCGGCTTACT 1021 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA GAATATATAC 1081 TGATATGTAT ACCCGAAGTA TGTCAAAAAG AGGTATGCTA TGAAGCAGCG TATTACAGTG 1141 ACAGTTGACA GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT ATCTCCGGTC 1201 TGGTAAGCAC AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC TGGAAAGCGG 1261 AAAATCAGGA AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC TCTTTTGCTG 1321 ACGAGAACAG GGGCTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT 1381 TATCGTCTGT TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATGGTG 1441 ATCCCCCTGG CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG 1501 GTGCATATCG GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC 1561 TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC 1621 ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG CCAGTCTGCA 1681 GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG 1741 CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTCTT 1801 GTACAAAGTG GTTGATCCTC TCGAGCCTCT AGAACTATAG TGAGTCGTAT TACGTAGATC 1861 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA 1921 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA 1981 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT 2041 GGGAGGTTTT TTAATTCGCG GCCGCGGCGC CAATGCATTG GGCCCGGTAC CCAGCTTTTG 2101 TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 2161 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 2221 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 2281 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 2341 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 2401 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 2461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 2521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 2581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 2641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 2701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 2761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 2821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 2881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 2941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 3001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 3061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 3121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 3181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 3241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 3301 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 3361 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 3421 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 3481 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 3541 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 3601 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 3661 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 3721 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 3781 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 3841 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 3901 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 3961 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 4021 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 4081 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 4141 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 4201 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 4261 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTAAATTG TAAGCGTTAA TATTTTGTTA 4321 AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC 4381 AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG 4441 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT 4501 CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC 4561 CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG 4621 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG 4681 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA 4741 CAGGGCGCGT CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG 4801 GCCTCTTCGC TATTACGCCA GTCGACCATA GCCAATTCAA TATGGCGTAT ATGGACTCAT 4861 GCCAATTCAA TATGGTGGAT CTGGACCTGT GCCAATTCAA TATGGCGTAT ATGGACTCGT 4921 GCCAATTCAA TATGGTGGAT CTGGACCCCA GCCAATTCAA TATGGCGGAC TTGGCACCAT 4981 GCCAATTCAA TATGGCGGAC TTGGCACTGT GCCAACTGGG GAGGGGTCTA CTTGGCACGG 5041 TGCCAAGTTT GAGGAGGGGT CTTGGCCCTG TGCCAAGTCC GCCATATTGA ATTGGCATGG 5101 TGCCAATAAT GGCGGCCATA TTGGCTATAT GCCAGGATCA ATATATAGGC AATATCCAAT 5161 ATGGCCCTAT GCCAATATGG CTATTGGCCA GGTTCAATAC TATGTATTGG CCCTATGCCA 5221 TATAGTATTC CATATATGGG TTTTCCTATT GACGTAGATA GCCCCTCCCA ATGGGCGGTC 5281 CCATATACCA TATATGGGGC TTCCTAATAC CGCCCATAGC CACTCCCCCA TTGACGTCAA 5341 TGGTCTCTAT ATATGGTCTT TCCTATTGAC GTCATATGGG CGGTCCTATT GACGTATATG 5401 GCGCCTCCCC CATTGACGTC AATTACGGTA AATGGCCCGC CTGGCTCAAT GCCCATTGAC 5461 GTCAATAGGA CCACCCACCA TTGACGTCAA TGGGATGGCT CATTGCCCAT TCATATCCGT 5521 TCTCACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC ACTTGGCAGT ACATCAATAT 5581 CTATTAATAG TAACTTGGCA AGTACATTAC TATTGGAAGG ACGCCAGGGT ACATTGGCAG 5641 TACTCCCATT GACGTCAATG GCGGTAAATG GCCCGCGATG GCTGCCAAGT ACATCCCCAT 5701 TGACGTCAAT GGGGAGGGGC AATGACGCAA ATGGGCGTTC CATTGACGTA AATGGGCGGT 5761 AGGCGTGCCT AATGGGAGGT CTATATAAGC AATGCTCGTT TAGGGAAC //
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