E.coli GT115大肠杆菌菌株-感受态细胞-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

E.coli GT115大肠杆菌菌株-感受态细胞

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Description

GT115 is a strain of E. coli that has been specifically engineered to support the growthof plasmid DNAs harboring the R6Kg origin of replication and carrying hairpinstructures, such as pCpG-mcs, pCpG-LacZ and pCpG-siRNA vectors. GT115 has thepir gene that encodes the p protein which is required by vectors utilizing theR6Kg ori. Hairpin structures are known to be unstable in E. coli due to theirelimination by a protein complex called SbcCD that recognizes and cleaveshairpins1 . To increase their stability in E. coli, we developed GT115 bydeleting the sbcC and sbcD genes. This modification significantly improves thenumber of recombinant clones harboring a plasmid with hairpin structures. Thisstrain contains the rpsL (StrA) gene which confers resistance to streptomycin.

GT115 Genotype:

F- mcrA ∆(mrr-hsdRMS-mcrBC)f80lacZDM15 ∆lacX74 recA1 rpsL (StrA) endA1 Ddcm uidA(DMluI)::pir-116 ∆sbcC-sbcD