pXNGW植物BiFC双分子荧光互补载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pXNGW植物BiFC双分子荧光互补载体

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

35S启动子，目的基因C端带有YFP 1-172片段，N端6xHis标签，Gayeway技术兼容。

BiFC System info:

This Bimolecular Fluorescence Complementation system is based on the 172

N-terminal amino acids of EYFP, and the 85 C-terminal aa.s of ECFP (aa.s 154-239).

Use of the 4 different vectors yields C- and N-terminal fusions of a putative interactor

protein to both of the of the fluorescent protein halves.

All 4 vectors are binary with a backbone derived from pPZP312, a single 35S promoter

and a CaMV terminator (derived from pRT100).

The vectors confer Spectinomycin resistance in E. coli and Agrobacterium, and Basta

resistance in plants.

All vectors contain a 6xHis-tag allowing for simple affinity-based purification of the

expressed proteins.

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