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pELPs 19-BB-z plasmid
BioVector NTCC中国质粒载体菌种细胞基因保藏中心
pELPs 19-BB-z临床级CAR-T慢病毒质粒,水泡性口炎病毒G蛋白假型慢病毒质粒,EF1a启动子,表达anti-CD19 scFv,人CD8a铰链和跨膜结构域,人4-1BB和CD3zeta信号结构域。
A vesicular stomatitis virus protein G pseudotyped clinical grade lentiviral vector (designated pELPs 19BBz) directing expression of anti-CD19 scFv derived from FMC63 murine monoclonal antibody, human CD8a hinge and transmembrane domain, and human 4- 1BB and CD3zeta signaling domains was produced. Constitutive expression of the transgene was directed by inclusion of an EF- l a (elongation factor- l a promoter); LTR, long terminal repeat; RRE, rev response element. (cPPT) and the central termination sequence (CTS).
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